CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
Cellular activity of Wharton's Jelly-derived mesenchymal stem cells on electrospun fibrous and solvent-cast film scaffolds
Authors
M. Azami
Z. Bagher
+3 more
S. Ebrahimi-Barough
M.T. Joghataei
M. Safa
Publication date
1 January 2016
Publisher
Abstract
It was shown that topography and surface chemistry of materials influence cell behaviors. In this study, the effects of chemistry and topography of scaffold surface on adhesion, proliferation and differentiation of Wharton's Jelly mesenchymal (WJMSCs) stem cells into motor neurons were investigated. WJMSCs were cultivated in an neurogenic inductive medium on the surface of modified and unmodified polycaprolactone (PCL) electrospun fibrous and solvent-cast film scaffolds. All the scaffolds were characterized according to their ability to support cell attachment and viability by SEM and MTT assay. The expression of motor neuron-specific markers was assayed by real-time PCR after 15days post induction. Results showed that attachment, proliferation and differentiation of WJMSCs into motor neuron-like cells on the nanotopographic surface was higher than that of the cells on the solvent-cast scaffolds. In addition, regardless of their topography, WJMSCs cultured on collagen-coated PCL nanofibrous showed results similar to collagen-coated PCL films. Results suggested that surface chemistry has more impact on WJMSCs behaviour rather than topography. In conclusion, collagen-coated electrospun PCL have potential for being used in neural tissue engineering because of its bioactive and three-dimensional structure which enhance viability and differentiation of WJMSCs. © 2015 Wiley Periodicals, Inc
Similar works
Full text
Available Versions
eprints Iran University of Medical Sciences
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:eprints.iums.ac.ir:4097
Last time updated on 10/10/2019