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Molecular characterization of hepatitis D virus genotypes circulating in Iran
Authors
S.M. Alavian
N. Ansari
+6 more
M. Farahani
H. Keyvani
P. Meysami
S.H. Monavari
S.-H. Mozhgani
M. Taghizadeh
Publication date
1 January 2018
Publisher
Abstract
Aim: To determine the molecular epidemiology and characterization of hepatitis D virus (HDV) genotypes circulating in different provinces of Iran. Patients & methods: In this study, the presence of HDV RNA was tested in sera that were positive for hepatitis B surface antigen and HDV antibody by nested-PCR. HDV genotypes were subsequently analyzed using restriction fragment length polymorphism (RFLP) assay and then confirmed by sequencing. Results: 86.5 of positive PCR patients had genotype I and 8.1 had genotype II while the genotype of 5.4 of the patients remained undetermined by RFLP. Sequencing followed by phylogenetic analysis demonstrated that all the Iranian isolates were from genotype I. Conclusion: Although analyzing the RFLP of RT-PCR is a simpler method, the gold standard of genotyping of HDV is the phylogenetic analysis based on sequencing. © 2018 Future Medicine Ltd
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eprints Iran University of Medical Sciences
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oai:eprints.iums.ac.ir:998
Last time updated on 10/10/2019
eprints Iran University of Medical Sciences
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:eprints.iums.ac.ir:7063
Last time updated on 10/10/2019