Shipley, Scott T.

NASA Technical Reports Server

Spectrum
ISS Fluorescence Imaging
ISS R&D 2019
Spectrum Payload Development Team
Kennedy Space Center
1
https://ntrs.nasa.gov/search.jsp?R=20190028814 2019-09-26T19:36:31+00:00Z
Science & Engineering Goals
Building on ABRS Heritage
ISS022-E-011304 (15 Dec. 2009) - NASA astronaut Jeffrey Williams, Expedition 22 
commander, conducts a daily status check of the Advanced Plant Experiments on 
Orbit (APEX) experiment in the Kibo laboratory of the International Space Station. 
During each check, Williams looks for health and color of the plants, since the 
Cambium plants are removed from the Advanced Biological Research System (ABRS). 
Evolution of ABRS into Spectrum
ABRS Spectrum
Fluorescence GFP CFP, GFP, YFP, OFP, RFP, Chlor
Imaging Video RGB Color Imaging
Subjects One Petri Four Petri/Microtiter
Camera Bionetics Illunis 71 Mpix (13 µm)
2
Spectrum Flight Unit
Science Verification Test (SVT)
Excitation Light Source
Carousel with Petri and
Microtiter Plates Visible
Air Filter Cartridge
Filter Wheel
Grow Light Source
3
Fluorescence Imaging
10-day ISSES Chamber SVT
GFP
RFP
4Arapadopsis exhibiting GFP and RFP Fluorescence on SVT Day 10
Color Imaging
Bayer Filters with White LED
5
Color Animation
Scheduled Hourly Imaging
0100Z
6
Link to 
mp4
Oribtal Replaceable Units (ORU)
PI Provision Components
Illunis
71 Mpix
Camera Pe
tr
i D
is
h
7
Canon
EF Lens
Fi
lt
er
 W
h
e
el
Em
is
si
o
n
 F
ilt
er
s
Air Filter Cartridge
Carousel
Assy
Sensor
PCB
Spectrum Flight Unit ORU
8
Excitation Light Source ORU
NASA NTR KSC-14104
9
Fastener
Housing
Edge Light 
Blocker
Diffuse
r
Color Filter
Quad 10 mm 
Optic LED 
Board
RTV Seal
Connector
Retaining 
Ring
RPC Photonics 40 degree
Engineered Diffuser on Polycarbonate
Carclo
Optic
Bandpass
Filter
LED Quad
Collimation is required to achieve spectral bandpass
when LED light is passing through “Color Filter”
I , 
! 
I 
I 
I " I 10 
En0g,~~s':r~ Rl-'C 1-'botom CJ ,. ., 
r&•s-A- •R 
£o:;~~~l..'j 190- 4 ., ,,;,;uiiu,Ii',111 ,111• 111111 
LOI 1,111i1ilflll\:1t11 1111 
w,o ;~: ; ; , 1, 11 
Excitation Light Source ORU
ELS Uniformity
10
Zemax Analysis
• ].; NSC Shad~ Model - - )( 
X 
-------- 1 00 mm 1 
Excitation & Emission Filters
COTS Filter Selections
Protein 
Categories
Excitation 
(nm)
Excitation Filter
Center
Wavelength/Band 
Pass (nm)
Dichromatic
Mirror
Cut-On (nm)
Emission 
(nm)
Barrier Filter
Center 
Wavelength/Band 
Pass (nm)
Cyan ECFP-
type
~439 435 / 40 460 LP ~476 495 / 50
Green EGFP-
type
~484 470 / 40 495 Long 
Pass (LP)
~507 515 / 30
Yellow EYFP-
type
~514 490 / 40 515 LP ~527 540 / 30
Blue EBFP-
Type
~383 375 / 50 405 LP ~445 445 / 50
Orange ~548 525 / 40 550 LP ~562 585 / 50
Red ~584 560 / 55 590 LP ~607 630 / 60
Chlorophyll ~484 470 / 40 495 LP ~680 680 / 50
SRD 3.1.2 Spectrum shall provide fluorescence imaging of (1) emission wavelength at a time. Common proteins and their excitation and emission 
wavelengths are listed in the table. Note: The proteins and their associated wavelengths are examples and are not requirements; actual proteins and 
wavelengths to be imaged shall be determined during pre-mission testing by the appropriate Principal Investigator (PI).
11
Fi ~er· !Description E~dtafon IE1mission M1neumonic Wh,eell filte1r [nm]I !Filter (nm] Color 
1 Bay,er Blue· 458/64, 
2 Bay,er Gre,en 515/50 
3 Bay,er R.ed 650/100 
4 "franspanmt 409/lP' White 
s CFP 435/40 482/25 
6 ·'' GFP 466/4i0 512/25 Gree 
7 YFP 504/12 539/30 Yellow 
8 OFP 534/20 585/40 
9 RFP 572/28 629/56 
. CFP 5. OF 6.C LO OP y 1Qi CHLOR CFP ,&
1GFP 680/42 
Spectrum Optical Geometry
PI Technical Reference
5 cm
X = 72mm
D
f
3
2
m
m
 d
ia
x 
2
m
m
4
2
m
m
 d
ia
2
1
m
m
 d
ia
f/2.8
10000 (H) x 7096 (V)
3.1 x 3.1 um pixel
31mm (H) x 22mm (V)
12
N
e
ar
 F
ie
ld Far Field
Depth of field
l_// 
Carousel ORU
Challenge to Suppress Fogging
13
Control Petri’s held in normal storage
Carousel ORU
Method to Suppress Condensation
Front
21.8 C
21.4 C
Back
14
TEC Cooling
........ " . 
. . . . . . . . ' . 
• I • • I • I • f 
,, ....... . 
• • I r • • • • • • • • 
t I I • I • ft I f • • 
···•· ..... ,., 
------~ --- --- -
.... .J;:::s::--------~=--=-~ -
2S 
20 
2H 
2•. 
24 2 
24 
23 8 
2~6 
23..<I 
21.2 
29 
221 
226 
~ 2J• !m 
2l 
21.8 
2J.6 
'1 .• 
2U 
21 
Petri 2 Temp Data : With Carousel Ducting 
1 ·4 l :s.J 141)3 14:13 14:23 l4 14 4 l•.S3 1.5:03 1S.ll lS.ll IS:33 15 43 15:51 16;01 16 13 1123 16:ll 16:43 16.Sl 17:03 17:13 17.23 17:33 17.4 17.!>l 
Baclt Petr12 --Front ~ tr12 amb rAlr 
Carousel ORU
Condensation Suppression Test
15
Environmental Controls
CO2, H2O vapor, Temperature, VOC
ISS Source
16
ISS Sink
ERC SourcePetri &
Microtiter
LiOH Sink
Chemsorb® Sink
ERC Sink
CO2 increase Mode 
-~.• 
..... 
TC8 
.) 
TEC Heat Exchanger Fin Temp 
(behind cover) 
Air Filtaration ModH l & 2 
:. 
;I 
\ 
Carbon Dioxide Management
ISSES Chamber at 4000 ppm
17
SPECTRUM Chamber CO2. 
4500 
4000 
3500 
Stop CO2 injection 
3000 
Start CO2 filt,er 
- 2500 E 
a. 
a. 2000 ......... 
N 
0 1500 u 
1000 
500 
Start CO2 injecti,on 
0 
0 so 100 150 200 250 
Time [minutes] 
Unique Carousel ORU
“The Tomko”
18PI Provision Carousel for 100 mm Macro Imaging
Canon EF Lens ORU
100 mm USM
19
20
Questions?
Dr. Scott T. Shipley, Spectrum Test Lead
Lasso Contract, Kennedy Space Center
scott.t.shipley@nasa.gov


Spectrum: ISS Fluorescence Imaging

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