Selection of codominant markers for investigation of genetic diversity of Echinocystis lobata (Michx.) Torr. et A. Gray populations

Abstract

Study was funded by Lithuanian Research Council, Grant number No. SIT-02/2015Wild cucumber (Echinocystis lobata) – climbing annual herbaceous plant, growing in coastal habitats, riverbanks, lakesides, wetlands and wastelands. It is native in North America, but in many European countries is recognized as invasive species. Within last two decades E. lobata is very intensively spreading in Lithuania and is considered to be one of the most aggressive invasive plants. Until now there is a lack of information about genetic diversity of Wild cucumber populations. Simple Sequence Repeats (SSR) markers appeared to be the most often used markers for understanding relations between invasive species and components of natural ecosystems. The objective of present study was to select codominant SSR markers for evaluation of genetic diversity of Echinocystis lobata populations. The DNA was extracted by DNA Purification Kit. Thirty SSR markers (N1, N6, N12, S9, S13, S15, S26, SSR10018, SSR05723, SSR16695, SSR16226, SSR23220, SSR22653, SSR23370, SSR01738, SSR16056, SSR05012, SSR05125, SSR07543, SSR19998, SSR16068, SSR02895, SSR20852, SSR31399, SSR20218, SSR29620, SSR14861, SSR11340, SSR11043) were tested for buffer type, temperature and other conditions of DNA amplification. DNA fragments were evaluated after electrophoresis on 1.5 % agarose gel. In our study seventeen (N6, N12, S9, S13, S15, S26, SSR05723, SSR23220, SSR23370, SSR05012, SSR07543, SSR19998, SSR16068, SSR31399, SSR20218, SSR29620, SSR11340) out of thirty markers showed positive resultsBiologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta