Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte

Abstract

The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease in the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved