In order to investigate the serological response to Chlamydophila psittaci in birds, we evaluated the presence of neutralizing antibodies against C. psittaci in 250 sera of pigeons collected in Emilia-Romagna Region (Northern Italy) and we have correlated the results to those obtained by microimmunofluorescence test (MIF). C. psittaci antibody prevalence detected by MIF was 40%. Neutralizing antibodies were specific for C. psittaci and were present in 22 out of 100 MIF positive sera