Aflatoxin contamination of milk was an emerging problem in Italy in Autumn 2003 and has represented an important risk factor for consumers. The European Union (EU) has established regulatory limits of the presence of AfM1 in milk and has suggested the methods of control: immunoassay tests (ELISA) for screening and high performance liquid chromatography (HPLC) for confirmation. Hence the importance to constantly control the performances of analytical methods. The aim of this research was to evaluate the performances of two ELISA kits, produced by different suppliers, on naturally contaminated raw milk in a range from 10 to 85 ng/L. The samples were previously analysed by HPLC to determine the level of AfM1. Results showed good responses in both kits in terms of precision. In addition to that some trials have been conduced to investigate the possible influence of time on the response of kits ELISA
