CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
Frequency of Toxoplasma gondii in Pork Meat in Ocotlan, Jalisco, Mexico
Authors
D. Correa
R. Franco
+7 more
M.L. Galvan-Ramirez
H. Luna-Pasten
A.L. Madriz Elisondo
L.R.R. Perez
A.R. Rincon-Sanchez
A. Salazar-Montes
C.P.R. Torres
Publication date
1 January 2010
Publisher
Universidad de Guadalajara (UDG)
Abstract
Toxoplasmosis is an infection caused by Toxoplasma gondii, an intracellular obligate parasite. Its transmission has usually been attributed to ingestion of undercooked or raw meat. The frequency of T. gondii in pork, the most common meat for human consumption in Jalisco, Mexico, is unknown; in Guadalajara city high prevalence of human toxoplasmosis has been documented. Forty-eight samples of pork meat from butcher shops in Ocotlan city were analyzed. Through bioassay, 50 g of tissue was homogenized in an acidic pepsin solution and inoculated subcutaneously to previously immunosuppressed mice. Blood samples from the mice tail vein were obtained before inoculation and 7, 14, 28, and 45 days postinoculation to analyze anti-Toxoplasma immunoglobulin (Ig) M and IgG antibody kinetics by indirect enzyme-linked immunosorbent assay. For histopathology, small fragments of the brain, lungs, heart, and skeletal muscle were extracted on day 45 and were stained with hematoxylin and eosin. Also, DNA was extracted from the pork meat for PCR amplification of the Bl gene. Even though all pork samples were negative by histopathology and PCR, IgG and IgM antibodies against T. gondii were detected in 1 of the 48 inoculated mice, reflecting a frequency of 2.1% positive pork meat, which is lower than expected but similar to that found in other regions. Copyright © International Association for Food Protection
Similar works
Full text
Available Versions
Red Mexicana de Repositorios Institucionales
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:riudg.udg.mx:20.500.12104/...
Last time updated on 03/09/2019