Non-Peer ReviewedNatural 13C abundance was measured in two Gleysols, one from a forest and one which had been under continuous corn for more than 25 yr. Delta 13C values increased slightly with depth in the forest soil. In the corn soil, delta 13C values were similar throughout the plow layer but lowest in the deepest layer sampled, 30-50 cm. Between 29 and 3 5% of the carbon in the plow layer of the cultivated soil was derived from corn. The 0-5 cm layer of each soil was separated into particle size fractions; delta 13c values indicated that the sand fraction had the largest proportion of carbon derived from corn whereas the coarse silt had the smallest proportion