Germida, J.J.

Helgason, B.L.

Kuzmicz, S.C.

English

University of Saskatchewan Research Archive

1S.C. Kuzmicz, 1B.L. Helgason and 2J.J. Germida 1Agriculture and Agri-Food Canada, Research Centre, Saskatoon, Saskatchewan, Canada;2Department of Soil Science, University of Saskatchewan, Saskatoon, Saskatchewan.   Microbial biodiversity supports key functions (e.g. N cycling) necessary  for sustained productivity in agroecosystems.  Long-term crop production may select for management-specific communities that actively perform biogeochemical cycling.  We are studying the cumulative effects of 100 years of wheat production on  soil microbial communities (SMC) in a long term soil experiment (LTSE) at Lethbridge, AB.  100 years of farming: long-term differences in cropping intensity and N fertilization impact soil microbial community structure Materials and Methods Results Acknowledgements Objectives Summary  To determine effects of 100 years of varied cropping intensity (CI) on soil microbial abundance and community structure.  To study the impact of 45 years of N fertilizer application on soil microbial abundance and community structure. • Abundance of broad microbial functional groups were differently affected by both long-term cropping intensity and N fertilizer. • PLFA profiles provided an overview of the SMC structure in response to management illustrating that agricultural practices have resulted in divergence of microbial populations that drive biogeochemical cycling in agroecosystems. • Management changes influenced inorganic N concentration.  The authors would like to thank Agriculture and Agri-food Canada for funding. Also thanks to colleagues at the Lethbridge Research Center  and Panchali Katulanda for their generous help with Rotation ABC. © 2013  Figure 1: Rotation ABC Plot layout. Figure 2: Microbial biomass for RA and RC with +/- N. • Total biomass was affected by the interaction of fertilizer with rotation (p<0.02). This pattern is echoed in the bacterial and fungal markers, however no significant interactions were detected. • Both bacterial (p<0.01) and fungal (p<0.02) biomass were higher in fertilized than unfertilized soils.           Total    Bacterial       Fungal  Table 1: Quantities of gram positive (Gr+), gram negative (Gr-) bacteria, arbuscular mycorrhizal fungi (AMF) and stress PLFA markers.  Table 3: General soil characteristics at the time of sampling (fall 2012).  Figure 3: Ordination (NMDS) of  microbial PLFA  community structure. • Microbial community structure was most distinct in the fertilized continuous wheat, compared to the other treatments. • Unfertilized continuous wheat and fertilized W-W-F were similar.  Rotation ABC at Lethbridge is situated on a Orthic Dark Brown Chernozem (MAP 386mm, MAT 5.7°C). The main plot factor is Rotation and sub-plot factor is fertilizer (five pseudoreplicated transects).  Rotation A (RA): continuous wheat (W)   Rotation C (RC): (W-W-F) all phases all years. Second year wheat was selected (W-W-F; C1 in 2012) to de-emphasize the short term effects of the fallow phase on microbial abundance.   N (ammonium nitrate) fertilizer treatments :   0 kg ha-1 (-N)   45 kg ha-1 (+N)   Soil sampling occurred post harvest in  September 2012. Four  10.0 cm depth  cores  with 3.75 cm diameter were collected for each replicate point along the transects.  Microbial phospholipid fatty acids (PLFA) were extracted from 4.0 g of freeze dried soil. Samples were quantified using an internal standard (19:0) and identified using GC-FID and the MIDI TM  system. Microbial abundance data were analyzed using ANOVA in SPSS v.20 and community profiles were subjected to non-metric multidimensional scaling (NMDS) ordination in PCOrd v.5.0.  Soil pH and inorganic nutrients N, P, K and S were determined by  ALS Environmental lab (Saskatoon, Saskatchewan).         Additional work  Fertilizer  pH  N P K S      mg kg-1 soil Rotation A -N  7.5 (0.1)  4.0 (1.4) 3.3 (1.0) 476  (51) 41.8 (35.1) (contin. wheat) +N  6.7(0.4)  11.3(3.4) 4.4(1.7) 677 (161) 10.9 (2.2) Rotation C -N  7.8(0.1)  4.8(0.8) 4.4(0.9) 420 (30) 14.5 (1.6) (W-W-F) +N  7.4(0.5)  4.7(0.8) 4.5(0.4) 502 (62) 8.1 (0.8) ANOVA R  p<0.01  p<0.01 p<0.20 p<0.03 p<0.11  F  p<0.01  p<0.01 p<0.26 p<0.01 p<0.06  RxF  p<0.21  p<0.01 p<0.15 p<0.22 p<0.19  • Nitrogen fertilizer significantly increased the abundance of both Gram positive and Gram negative bacteria.  • Rotation and fertilizer altered both pH and K in the soil.  • Available N was influenced by an interaction of rotation with fertilizer. Results Abundance of major soil microbial functional groups Soil microbial community structure  The abundance of N cycling functional gene abundance will be investigated with real-time PCR. Archived soils, dating back to 1910 will be used to  study changes in soil bacterial and fungal diversity over time with 16S rRNA  and ITS targeted 454 pyrosequencing.  Fertilizer  Gr+  Gr- AMF Stress 1 Stress 2    nmol PLFA g-1 soil Rotation A -N  2.5(0.4)  4.3(0.7) 0.8(0.2) 0.4(0.0) 0.3(0.0) (contin. wheat) +N  5.5(3.2)  12.0(6.0) 3.8(4.5) 0.4(0.2) 0.3(0.1) Rotation C -N  2.4(0.2)  4.0(0.4) 0.8(0.1) 0.4(0.0) 0.2(0.1) (W-W-F) +N  3.3(0.7)  5.6(1.8) 1.0(0.2) 0.4(0.0) 0.3(0.1) ANOVA R  p<0.18  p<0.06 p<0.22 p<0.99 p<0.46  F  p<0.03  p<0.01 p<0.17 p<0.91 p<0.10  RxF  p<0.22  p<0.08 p<0.26 p<0.55 p<0.88  Introduction 

100 years of farming: long-term differences in cropping intensity and N fertilization impact soil microbial community structure

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100 years of farming: long-term differences in cropping intensity and N fertilization impact soil microbial community structure

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