Arcand, M.

Bazghaleh, N.

Bell, J.

Helgason, B.

Lamb, E.

Mamet, S.D.

Morales, Z.

Parkin, I.

Siciliano, S.D.

Taye, Z.

Vail, S.

Williams, S.

English

University of Saskatchewan Research Archive

Dynamics of root-associated fungal community of canola genotypes in Saskatchewan soils1Bazghaleh, N., 1Mamet, S.D., 1Bell, J., 1Morales, Z., 1Taye, Z., 1Williams, S., 1Arcand, M., 1Lamb, E., 2Parkin, I., 1Siciliano, S.D., 2Vail, S., and 1Helgason, B.1Department of Soil Science, University of Saskatchewan2Agriculture and Agri-Food Canada Saskatoon Research CentreCanola• World's most important oilseed crop• Canola oil is renowned for its nutritional qualities and industrial applications• It is recommended to grow canola 1 year in 4 on the same field• Canola is susceptible to several diseases• Breeders continue to develop varieties with increased resistance to diseasesImportance of canola root microbiomeProfitable canola production relies heavily on adequate plant nutritionCanola root system: root health and nutrient uptake capacityCanola root microbiome: diversity, composition and dynamicsRhizosphereBakker et al., 2018Canola: 8 diverse genotypes (AAFC-NAM pop.)Location: Llewellyn, SKYears: 2016 & 2017Sampling: 10 times - weeklyMaterial: Root and RhizosphereAnalysis: DNA sequencing of fungal microbiome using universal fungal primers (ITS1-F / ITS2) on Illumina Miseq platformDADA2: Fungal community analysis: Single nucleotide variants (SNVs)ExperimentDominant fungal genera in canola rhizosphereFungal genera in canola rhizosphere over the growing season Dominant fungal genera in canola rootsOlpidium brassicae in canola rootsFungal genera in canola roots over the growing season Fungal microbiome in roots of canola genotypesDf SumsOfSqs MeanSqs F.Model R2 Pr(>F)Genotype 7 2662 380.3 1.2778 0.01823 0.004**Year 1 8967 8966.5 30.1322 0.06141 0.001***Genotype : Year 7 2556 365.2 1.2271 0.01751 0.013*Residuals 443 131825 297.6 0.90285Total 458 146009 1Fungal microbiome in canola roots over the growing seasonDf SumsOfSqs MeanSqs F.Model R2 Pr(>F)Genotype 7 2662 380.25 1.2077 0.01823 0.012*Week 1 1651 1651.1 5.2439 0.01131 0.001***Genotype : Week 7 2213 316.15 1.0041 0.01516 0.452Residuals 443 139483 314.86 0.9553Total 458 146009 1Fungal microbiome in the rhizosphere of canola genotypesDf SumsOfSqs MeanSqs F.Model R2 Pr(>F)Genotype 7 4446 635 1.649 0.02045 0.002**Year 1 36618 36618 95.053 0.16844 0.001***Genotype : Year 7 4135 591 1.533 0.01902 0.006**Residuals 447 172202 385 0.79209Total 462 217401 1Fungal microbiome in the rhizosphere of canola over the growing seasonDf SumsOfSqs MeanSqs F.Model R2 Pr(>F)Genotype 7 4446 635.1 1.394 0.02045 0.025*Week 1 6586 6586.1 14.4557 0.03029 0.001***Genotype : Week 7 2711 387.3 0.8501 0.01247 0.878Residuals 447 203657 455.6 0.93678Total 462 217401 1Summary ◦ Canola genotype and environmental conditions shape the root fungal microbiome ◦ Root fungal microbiome changes over the growing season◦ Environmental conditions can override the effect of genotype on the composition of root microbiome AcknowledgementsDr. Bobbi HelgasonDr. Steven Siciliano Charlotte NorrisAlix Schebel summer students: Lauren Reynolds, Cordell VanGenderen, Yolanda Iannucci and Kyra Blomquist, and field crews.This research was funded by a grant from the Plant Phenotype an Imaging Research Centre which is supported by the Canada First Research Excellence Fund from the Natural Sciences and Engineering Research Council. 

Dynamics of root-associated fungal community of canola genotypes in Saskatchewan soils

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Dynamics of root-associated fungal community of canola genotypes in Saskatchewan soils

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