Estimation of genetic diversity and indentity in plus tree clones of Norway spruce (Picea abies (L.) Karst) using RAPD method

Abstract

The RAPD (random amplified polymorphic DNA) was used to assess genetic diversity and to identify genotype specific markers in Norway spruce (Picea abies (L.) Karst.) plus tree clones. Twenty-three genotypes originally collected from different localities of Lithuania and now growing in a clonal archive at Dubrava experimental-training forest enterprise were analyzed by using 20 oligonucleotide primers. A total of 299 RAPD bands were scored, of which 243 were polymorphic. The estimated genetic distance varied from 0.2905 to 0.5000. These results confirm that studied P. abies clones exhibit a high genetic variation. Fourteen genotype-specific DNA bands were identified. The RAPD technique was also used to check the clonal identity. We compared RAPD profiles of 5 ramets for each of 3 plus tree. Some differences in the RAPD patterns resulting from mis-planting or mis-labeling of cuttings were found. We also established a reference library of RAPD fingerprints for 23 clones, which could serve as a reference source in cases of litigation concerning clone identity. This study confirmed that the RAPD technique is a useful tool for Picea abies genetic analysis and for forestry service in managing propagation operations. Also it could be useful for Norway spruce genetic resources analysis and conservation in Lithuania