The identification, pathotype, distribution of golden potato cyst nematode (globodera rostochiensis) and status of pale potato cyst nematode (globodera pallida) in lithuanian agroecosystems

Abstract

Golden (Globodera rostochiensis) and pale (Globodera pallida) potato cyst nematodes are quarantine organisms. Potatoes are by far the most important host crop. Other Solanum spp. and their hybrids can also act as hosts. The aim of this study was to identify G. rostochiensis, G. rostochiensis pathotypes and distribution and also G. pallida status in soil samples from different field of different size and various forecrops in Lithuanian counties. Only G. rostochiensis was present in Lithuania after morphological identification and DNA polymerase chain reaction (PCR) which has been done in Lithuania for the first time. In small field areas up to 5 ha G. rostochiensis is more distributed than in bigger areas up to 30 ha. The presence of host plants in various forecrops, soil type – sands and sandy loams, also have influence on numerous distribution of G. rostochiensis. The experiment of G. rostochiensis pathotypes showed that only Ro1 pathotype is present in Lithuania