ElNaker, Nancy A.

Yousef, Ahmed F.

Yousef, Lina F.

ElNaker Nancy A.

Yousef Ahmed F.

Yousef Lina F.

University of Szeged

Trends in Natural Product Research – PSE Young Scientists’ Meeting Budapest, June 19th-21th, 2019 70  SL-23 doi: 10.14232/tnpr.2019.sl23  Antioxidant capacity and in vitro breast cancer cytotoxicity of aqueous extracts from Arthrocnemum macrostachyum are affected by drying method  Nancy A ElNaker, Ahmed F. Yousef and Lina F. Yousef  Department of Chemistry, Khalifa University, Masdar Campus, POBOX 54224, Abu Dhabi, UAE.  E-mail: nance.elnaker@ku.ac.ae  Arthrocnemum macrostachyum is a halophytic perennial shrub that is widespread across coastal zones in the Mediterranean basin, Middle East, and Asia.  This plant can survive in arid desert climates where temperatures can sometimes exceed 60 oC in mid-summer days. In this study, we examine how drying method (oven vs. lyophilization) and drying temperature (40 and 60 oC) of macerated extracts (water and 50% aq. ethanol) from A. macrostachyum are affected in terms of antioxidant capacity and bioactivity (cytotoxicity towards MDA-MB231 cancer cells). Oven drying caused a 1.5-2 fold reduction in antioxidant capacity for both extracts (water and 50% ethanol) compared to lyophilization. The lyophilized 50% ethanol extract had the highest antioxidant capacity (DPPH IC50=35.84 µg/mL) compared to all other treatments. Oven drying of extracts also resulted in a reduction in in vitro anti-cancer activity as measured using MTT assay on MDA-MB231 breast cancer cells. Lyophilized water extract showed the highest in vitro cytotoxic activity (LD50=0.671 mg/mL) compared to all other treatments. These findings indicate that drying conditions impact bioactivity and suggest that A. macrostachyum is a potential source of anti-cancer agents.   Acknowledgements This research is supported by a Khalifa University of Science and Technology grant award CIRA-2018-35.     

Antioxidant capacity and in vitro breast cancer cytotoxicity of aqueous extracts from Arthrocnemum macrostachyum are affected by drying method : [abstract]

http://acta.bibl.u-szeged.hu/62000/1/ysm_2019_070.pdf

Antioxidant capacity and in vitro breast cancer cytotoxicity of aqueous extracts from Arthrocnemum macrostachyum are affected by drying method

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