Choi, Hee-Jung

Ha, Ki-Tae

Park, Hye-Rin

Park, Mi-Ju

Park Hye-Rin

Choi Hee-Jung

Park Mi-Ju

Ha Ki-Tae

University of Szeged

Trends in Natural Product Research – PSE Young Scientists’ Meeting Budapest, June 19th-21st, 2019 96  PO-14 doi: 10.14232/tnpr.2019.po14  Paeoniflorin increases the adhesion of trophoblast to the endometrium by upregulating the expression of Integrin αVβ3 and αVβ5  Hye-Rin Park1,2,†, Hee-Jung Choi1,†, Mi-Ju Park1 and Ki-Tae Ha1,2*  1 Korean Medical Research Center for Healthy Aging and 2Department of Korean  Medical science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongsangnam-do, Republic of Korea † These authors contributed equally in this study.   *E-mail: hagis@pusan.ac.kr   Successful implantation requires uterine receptivity which is determined by diverse biological factors such as adhesion molecules, cytokines, growth factors, and receptors. In our previous study, water extract of Paeonia lactiflora enhanced embryo implantation in vitro and in vivo via induction of the leukemia inhibitory factor (LIF)-dependent expression of Integrin αVβ3 and αVβ5. To investigate which one is the major component, we performed high-performance liquid chromatography (HPLC) analysis. Next, we tested these five compounds to confirm whether these single compounds enhance the adhesion of human trophectoderm-derived JAr cells to the endometrial Ishikawa cells. In addition, we checked the expression of adhesion molecules in mRNA and protein levels and performed in vivo study using the implantation failure model mice by treating RU-486. Paeoniflorin, the most abundant molecule among tested five major compounds of P. lactiflora, showed enhancing effect on cellular interaction between JAr and Ishikawa cells. Paeoniflorin increased the expression of Integrin αVβ3 and αVβ5 in LIF-dependent manner. Furthermore, in vivo study showed that paeoniflorin significantly improved the number of implantation embryos. Therefore, our results suggest that paeoniflorin, a major compound of P. lactoflora, is a potent agent for enhancing endometrial receptivity.  Acknowledgements This study was supported by a National Research Foundation of Korea (RNRF) grant funded by the Ministry of Science, ICT & Future Planning (MISP), Korea Government.   

Paeoniflorin increases the adhesion of trophoblast to the endometrium by upregulating the expression of Integrin αVβ3 and αVβ5 : [abstract]

http://acta.bibl.u-szeged.hu/62025/1/ysm_2019_096.pdf

Paeoniflorin increases the adhesion of trophoblast to the endometrium by upregulating the expression of Integrin αVβ3 and αVβ5

Paeoniflorin increases the adhesion of trophoblast to the endometrium by upregulating the expression of Integrin αVβ3 and αVβ5

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