Butschek, Hannah

Gerstmeier, Jana

Jayasinghe, Lalith

Koeberle, Andreas

Lorenz, Sybille

Nagahawatte, Ajith

Napagoda, Mayuri

Qader, Mallique

Schneider, Bernd

Soyza, Sudhara De

Svatoš, Aleš

Werz, Oliver

Wijayaratne, Gaya Bandara

Napagoda Mayuri

Soyza Sudhara De

Gerstmeier Jana

Butschek Hannah

Lorenz Sybille

Qader Mallique

Nagahawatte Ajith

Wijayaratne Gaya Bandara

Schneider Bernd

Svatoš Aleš

Jayasinghe Lalith

Koeberle Andreas

Werz Oliver

University of Szeged

Trends in Natural Product Research – PSE Young Scientists’ Meeting Budapest, June 19th-21th, 2019 58  SL-11 doi: 10.14232/tnpr.2019.sl11  Plectranthus zeylanicus Benth: A potent source of secondary metabolites with antimicrobial, disinfectant and anti-inflammatory activities  Mayuri Napagoda1*, Sudhara De Soyza1, Jana Gerstmeier2, Hannah Butschek2, Sybille Lorenz3, Mallique Qader4, Ajith Nagahawatte1, Gaya Bandara Wijayaratne1, Bernd Schneider3, Aleš Svatoš3, Lalith Jayasinghe4, Andreas Koeberle2 and Oliver Werz2  1 Faculty of Medicine, University of Ruhuna, Galle 80000, Sri Lanka 2 Institute of Pharmacy, Friedrich-Schiller-University Jena, Philosophenweg 14, D-07743 Jena, Germany 3 Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745 Jena, Germany 4 National Institute of Fundamental Studies, Hantana Road, Kandy 20000, Sri Lanka  *E-mail: mayurinapagoda@yahoo.com  Plectranthus zeylanicus Benth is widely used in Sri Lankan folk medicine as a remedy for inflammatory and microbial diseases. In continuation of our previous work on anti-inflammatory activity in lipophilic extracts of this plant, the present investigation was undertaken to evaluate the antimicrobial and disinfectant potency in different organic extracts of P. zeylanicus and to isolate bioactive secondary metabolites. The activity-guided fractionation of the most potent dichloromethane extract resulted in the isolation of a pure compound which was extensively studied for its antimicrobial activity by broth microdilution method. Further, its disinfectant potency was evaluated by surface disinfectant assay, while the anti-inflammatory activity was determined by investigating its ability to inhibit the pro-inflammatory enzymes 5-lipoxygenase (5-LO) and microsomal prostaglandin E2 synthase (mPGES)-1. The spectral data revealed the identity of this compound as 7α-acetoxy-6β-hydroxyroyleanone. It displayed strong antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus aureus along with an extremely potent disinfectant capacity, which is comparable to the potency of a commercial disinfectant. Interestingly, it effectively inhibited 5-LO with IC50 of 1.3 and 5.1 µg/mL in cell-free and cell-based assay, respectively. Thus, the observed bioactivities of 7α-acetoxy-6β-hydroxyroyleanone rationalize the ethnomedicinal significance of P. zeylanicus.  Acknowledgements The financial support provided by the National Science Foundation, Sri Lanka is gratefully acknowledged.   

Plectranthus zeylanicus Benth : a potent source of secondary metabolites with antimicrobial, disinfectant and anti-inflammatory activities : [abstract]

http://acta.bibl.u-szeged.hu/61988/1/ysm_2019_058.pdf

Plectranthus zeylanicus Benth : a potent source of secondary metabolites with antimicrobial, disinfectant and anti-inflammatory activities

Plectranthus zeylanicus Benth : a potent source of secondary metabolites with antimicrobial, disinfectant and anti-inflammatory activities

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University of Szeged