Jánószky, Mihály

Lovas, Miklós

Patonay, Katalin

Pénzesné Kónya, Erika

Szalontai, Helga

Zámboriné Németh, Éva

Patonay Katalin

Szalontai Helga

Jánószky Mihály

Lovas Miklós

Pénzesné Kónya Erika

Zámboriné Németh Éva

University of Szeged

Trends in Natural Product Research – PSE Young Scientists’ Meeting Budapest, June 19th-21th, 2019 62  SL-15 doi: 10.14232/tnpr.2019.sl15  Main phenolic constituents of Mentha longifolia (L.) L. samples from Northern Hungary – extractability, variability and contribution to some in vitro antioxidant properties of the plant  Katalin Patonay1*, Helga Szalontai1, Mihály Jánószky1, Miklós Lovas1, Erika Pénzesné Kónya2 and Éva Zámboriné Németh3  1Food and Wine Research Institute, Eszterházy Károly University, Eger, Hungary 2Department of Botany and Plant Physiology, Eszterházy Károly University, Eger, Hungary 3Department of Medicinal and Aromatic Plants, Szent István University, Budapest, Hungary  *E-mail: patonay.katalin@uni-eszterhazy.hu   Mentha longifolia (L.) is a less studied wild-growing species. The aim of experiments is to define effective extraction of its polyphenolic antioxidants, and to observe the variability of them. The present work is the first phytochemical screening of the plant in Europe. Thirty-six accessions were sampled in flowering state. Soxhlet and ultrasonic (US) extraction, both with methanol (MeOH) and ethanol-water 7:3 (WA) were applied. HPLC-DAD measurements were performed on extracts. Dominant phenolics were rosmarinic acid (7043-38667 mg/kg drug, lower than determined in Israeli populations of the plant [1], similar to peppermint [2]) and hesperidin (from 1985 mg/kg to 20000< mg/kg), accompanied by diosmin (398.3-7987 mg/kg, similar to peppermint[2]), and cynaroside (318-2553 mg/kg). For cynaroside and rosmarinic acid, WA methods were more efficient than MeOH extractions. In case of the other two flavonoids, MeOH extraction were the more efficient compared with the WA and there was no significant difference between US and MeOH Soxhlet. Rosmarinic acid was in significant, medium correlation with the DPPH and FRAP values of extracts (R=0.49-0.55 depending on extract type; p<0.01 in all cases). Cynaroside did not show significant correlation with these activities contrary of its strong antioxidant capacity.  Other major constituents were observed in all of the chromatograms at ca. tR=13.7’ and tR=19.7’. Their identification needs further examinations.  Acknowledgements The authors gratefully acknowledge the valuable support of Bükk National Park in this project.  The research was supported by EFOP-3.6.1-16-2016-00001‚ Complex improvement of research capacities and services at Eszterhazy Karoly University.  References [1] Dudai N et al. Acta Hortic 2006; 709:69-78. [2] Guédon DJ, Pasquier BP. J Agric Food Chem 1994; 42:679-684.   

Main phenolic constituents of Mentha longifolia (L.) L. samples from Northern Hungary - extractability, variability and contribution to some in vitro antioxidant properties of the plant : [abstract]

http://acta.bibl.u-szeged.hu/61992/1/ysm_2019_062.pdf

Main phenolic constituents of Mentha longifolia (L.) L. samples from Northern Hungary - extractability, variability and contribution to some in vitro antioxidant properties of the plant

Main phenolic constituents of Mentha longifolia (L.) L. samples from Northern Hungary - extractability, variability and contribution to some in vitro antioxidant properties of the plant

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