Allard, Pierre-Marie

Wolfender, Jean-Luc

Wolfender Jean-Luc

Allard Pierre-Marie

University of Szeged

Trends in Natural Product Research – PSE Young Scientists’ Meeting Budapest, June 19th-21st, 2019 33 PL-10 doi: 10.14232/tnpr.2019.pl10 Do we still need to isolate Natural Products for their identification? - A paradigm shift in pharmacognosy.  Jean-Luc Wolfender*; Pierre-Marie Allard  School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU – Rue Michel Servet 1, 1211 Geneva 11, Switzerland  *E-mail: Jean-Luc.Wolfender@unige.ch  The recent rapid innovations made in metabolite profiling and bioassays may lead to a change of paradigm in NP research. Indeed having at hand full or partial of structure of possibly all metabolites in given natural extract open the possibility to perform pharmacognosy studies from a more holistic perspective. A question thus arise: do we still need to isolate Natural Products for their identification? The increasing amount of accurate metabolome data that can be acquired from massive sample sets, including high-resolution mass spectrometry analyses (data dependent HRMS/MS), allows natural extracts to be mapped to an unprecedented level of accuracy [1]. To this end, the creation of integrated databases linking the valuable published knowledge provided by pharmacognosy with recent metabolite profiling data through appropriate computer tools could be extremely useful for future development in NP research [2].  In this context we push forward our applications and further development of UHPLC-HRMS/MS molecular network (MN) approaches [3,4] to provide enhanced annotation confidence level though multiple scores, notably taxonomy and MN structural consistency, and we are benchmarking this. We will discuss how far we are from the unambiguous identification of NPs by these approaches and how such advances could significantly accelerate NP research in future. Various recent applications of our research in metabolomics and phytochemistry will illustrate these aspects.  Acknowledgements JLW is thankful to Swiss National Science Foundation (SNF) for supporting his metabolomics research projects (SNF 310030E-164289, 31003A_163424 and 316030_164095)  References [1] Wolfender JL et al. Anal. Chem. 2019; 91:704-742. [2] Allard PM et al. Curr. Opin. Biotechnol. 2018; 54:57-64. [3] Allard PM et al. Anal. Chem. 2016; 88:3317-23. [4] Allard PM et al. Curr. Opin. Chem. Biol. 2017; 36:40-49.   

Do we still need to isolate natural products for their identification? : a paradigm shift in pharmacognosy : [abstract]

http://acta.bibl.u-szeged.hu/61971/1/ysm_2019_033.pdf

Do we still need to isolate natural products for their identification? : a paradigm shift in pharmacognosy

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