Hassan, Kamal

Mohanraj, Rajesh

Venkataraman, Balaji

Mohanraj Rajesh

Hassan Kamal

Venkataraman Balaji

University of Szeged

Trends in Natural Product Research – PSE Young Scientists’ Meeting Budapest, June 19th-21st, 2019 85  PO-3 doi: 10.14232/tnpr.2019.po3  Nootkatone elicits hepatoprotective and anti-fibrotic actions in a murine model of liver fibrosis by suppressing oxidative stress, inflammation, and apoptosis  Rajesh Mohanraj1*, Kamal Hassan1,2 and Balaji Venkataraman1  1 Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, United Arab Emirates. 2 Department of Basic Sciences, College of Medicine and Health Sciences, United Arab Emirates University, United Arab Emirates.  *Email: rajm@uaeu.ac.ae  In this study, the hepatoprotective and anti-fibrotic actions of nootkatone (NTK) were investigated using carbon tetrachloride (CCl4)-induced liver fibrosis in mice. CCl4 administration elevated serum aspartate and alanine transaminases levels, respectively. In addition, CCl4 produced hepatic oxidative and nitrative stress, characterized by diminished hemeoxygenase-1 expression, antioxidant defenses, and accumulation of 4-hydroxynonenal and 3-nitrotyrosine. Furthermore, CCl4 administration evoked profound expression of pro-inflammatory cytokine expressions such as tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), and interleukin-1β (IL-1β) in hepatic tissues, which corroborated with nuclear factor-κB (NF-κB) activation. Additionally, CCl4-treated animals exhibited higher apoptosis, characterized by increased caspase 3 activity, DNA fragmentation, and poly (ADP-ribose) polymerase [PARP] activation. Moreover, histological and biochemical investigations revealed marked fibrosis in the livers of CCl4-administered animals. However, NTK treatment mitigated CCl4 -induced phenotypic changes. In conclusion, our findings suggest that NTK exerts hepatoprotective and anti-fibrotic actions by suppressing oxidative stress, inflammation, and apoptosis.  Acknowledgements This study was supported by intramural funds from the office of research and graduate studies, UAE University.   

Nootkatone elicits hepatoprotective and anti-fibrotic actions in a murine model of liver fibrosis by suppressing oxidative stress, inflammation, and apoptosis : [abstract]

http://acta.bibl.u-szeged.hu/62014/1/ysm_2019_085.pdf

Nootkatone elicits hepatoprotective and anti-fibrotic actions in a murine model of liver fibrosis by suppressing oxidative stress, inflammation, and apoptosis

Nootkatone elicits hepatoprotective and anti-fibrotic actions in a murine model of liver fibrosis by suppressing oxidative stress, inflammation, and apoptosis

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