MEKANISME ANTI TOXOPLASMOSIS EKSTRAK ETANOL KULIT MANGGIS (Garcinia mangostanaLinn) PADA MENCIT YANG DIINFEKSI Toxoplasma gondii

Abstract

Background:Toxoplasmosis is a dangerous infectious disease caused by Toxoplasma gondii in the blood or tissue. This disease is zoonotic which is a source of transmission from animals to humans. Therapy of herbal medicine for Toxoplasmosis can be determined by looking at IFN, IL-12 and MMP- 9expression, and seeing the level of organ damage. Purpose: to prove the mechanism of antitoxoplasmosis of mangosteen peel extract against Toxoplasma gondii. Methods: The study was divided into two stages. Stage I: determination of the effective dose 50% of mangosteen peel extract against Toxoplasma.gondii. Stage II: mechanism of antitoxoplasmosis of mangosteen peel extract against Toxoplasma gondii. This study used 50 mice divided into five groups: healthy control group (P0) given 0.5% CMC-Na, P1 group without treatment, P2 group given Cotrimoxazole 60mg/kgB, P3 and P4 groups given mangosteen peel extract 200 and 400 mg/kgBW (orally twice a day). After injection of Toxoplasmagondii 102 intraperitonially and given treatment for 5 days then blood samples taken for measurement of SGPT, SGOT, BUN and creatinine; liver and kidney organ samples to determine organ damage, IFN, IL-12 and MMP-9expression. Results: ED 50% at dose of 60mg/kgBW. Mangosteen peel extract 200mg/kgBW (P3) and 400mg/kgBB (P4) with significance (p<0.05) can reduce the number of parasites, levels of SGPT, SGOT, BUN and creatinine, reduce liver and kidney damage, increases IFN and IL-12 and decreases MMP-9 expression. Conclusion: Mangosteen peel extract 200mg/kgBW (P3) was proven as antitoxoplasmosis in mice can reduce the number of parasites, levels of SGPT, SGOT, BUN and creatinine, reduce liver and kidney damage, increases IFN and IL-12 and decreases MMP-9 expression