This research is conducted to identify the effects of immersion length and interval on Talinum paniculatum

(Jacq.) Gaertn adventitious roots’ biomass and saponin content in temporary immersion bioreactor. Immersion intervals used in this research are 3, 6, and 12 hours with immersion lengths 1, 3, 5, and 7 minutes. Adventitious roots were induced from leaf explants grown on MS medium added by IBA 2 mg/L. Two grams adventitious root are cultured in temporary immersion bioreactor with each treatment and kept for 28 days. Results of this research indicated different biomass and saponin content caused by each treatment. The best combination was found on immersion length of 5 minutes and 12 hours interval which resulting 3.67 g fresh weight, growth speed 0.027 g/day, spot area of saponin 12.56 mm2/0.01 g dry-weight, and spot area thickness scale 4+

Kristanti, Alfinda Novi

Manuhara, Yosephine Sri Wulan

Saputri, Nike Oktavia Sri

English

Universitas Airlangga Repository

    246   Scholars Academic Journal of Biosciences (SAJB)           ISSN 2321-6883 (Online) Sch.  Acad. J. Biosci., 2014; 2(4): 246-250                  ISSN 2347-9515 (Print) ©Scholars Academic and Scientific Publisher       (An International Publisher for Academic and Scientific Resources) www.saspublisher.com  Research Article  Production of Adventitious Root and Saponin of Talinum paniculatum (Jacq.) Gaertn. in Temporary Immersion Bioreactor Yosephine Sri Wulan Manuhara1*, Nike Oktavia Sri Saputri1, Alfinda Novi Kristanti2 1Biology Department, 2Chemistry Department Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia  *Corresponding author  Yosephine Sri Wulan Manuhara  Email:    Abstract: This research is conducted to identify the effects of immersion length and interval on Talinum paniculatum (Jacq.) Gaertn adventitious roots’  biomass and saponin content in temporary immersion bioreactor. Immersion intervals used in this research are 3, 6, and 12 hours with immersion lengths 1, 3, 5, and 7 minutes. Adventitious roots were induced from leaf explants grown on MS medium added by IBA 2 mg/L. Two grams adventitious root are cultured in temporary immersion bioreactor with each treatment and kept for 28 days. Results of this research indicated different biomass and saponin content caused by each treatment. The best combination was found on immersion length of 5 minutes and 12 hours interval which resulting 3.67 g fresh weight, growth speed 0.027 g/day, spot area of saponin  12.56 mm2/0.01 g dry-weight, and spot area thickness scale 4+.  Keywords: adventitious roots, saponin, temporary immersion bioreactor, Talinum paniculatum (Jacq.) Gaertn INTRODUCTION Talinum paniculatum (Jacq.) Gaertn. is a herb with many medicinal properties. In Indonesia T. paniculatum known as ginseng Jawa and it roots’  contains β-sitosteryl-β-D-glicosida  known as pharmaceutical products ingredient [1]. T. paniculatum adventitious root induction was successfully done using leaf explants on MS medium added by growth regulator substance IBA 2 mg/L. The disadvantage of using solid medium is that explants only absorb nutrition on the lower side which have direct contact with the medium, so that the result is less optimal compared to using liquid medium [2].   The use of liquid medium on plant tissue culture has some advantages. Liquid medium can provide homogenous culture condition, having faster growth rate, and relatively easy for sterilization [3-4]. However, there are several disadvantages of liquid medium mainly some technical issues such as hyperhydricity, cellular damage caused by propeller rotation when using bioreactor with churning system and oxygen deficiency [5]. Therefore, better methods to regulate aeration on the medium are needed. One of them is using temporary immersion applied to bioreactor known as Temporary Immersion Bioreactor (TIB) [6].   TIB is bioreactor which regulates nutrition and oxygen absorptions of the culture. In this condition, explants are not immersed in the medium all the time. There are several periods when the explants are not immersed [6].  When the explants are not immersed, they are free to absorb oxygen because of low oxygen solubility on immersed condition. Oxygen is needed by culture to unload energy provided by medium in form of sucrose. TIB immersion lengths and intervals can help explants growth [7]. One to fifteen-minute immersion length with 2-12 hours of immersion frequency also affects explants growth of perennial plants [3].   When culture is on immersed condition as a result of one-minute immersion length and 1, 12, 24-hour of immersion intervals on TIB, may cause stress on Hevea brasiliensis calluses identified by superoxide dismutase (SOD) [8]. Immersion length and interval in culture could cause  abiotic stresses. Abiotic stresses may affect production of  secondary metabolite contents [9]. Secondary metabolite synthesis, including saponin is a response to abiotic stress [10]. For example water stress treatment on T. paniculatum plant increase its saponin content on water 40% availability [11].  This research is conducted to identify the effects of immersion length and interval on biomass and saponin contents of T. paniculatum adventitious roots on liquid medium using temporary immersion system method on bioreactor.    

Production of Adventitious Root and Saponin of Talinum paniculatum(Jacq.) Gaertn. in Temporary Immersion Bioreactor.

    246 
 
 
Scholars Academic Journal of Biosciences (SAJB)           ISSN 2321-6883 (Online) 
Sch.  Acad. J. Biosci., 2014; 2(4): 246-250                  ISSN 2347-9515 (Print) 
©Scholars Academic and Scientific Publisher       
(An International Publisher for Academic and Scientific Resources) 
www.saspublisher.com 
 
Research Article 
 
Production of Adventitious Root and Saponin of Talinum paniculatum (Jacq.) 
Gaertn. in Temporary Immersion Bioreactor 
Yosephine Sri Wulan Manuhara1*, Nike Oktavia Sri Saputri1, Alfinda Novi Kristanti2 
1Biology Department, 2Chemistry Department 
Faculty of Science and Technology, Airlangga University, Surabaya, Indonesia 
 
*Corresponding author  
Yosephine Sri Wulan Manuhara  
Email:  
  
Abstract: This research is conducted to identify the effects of immersion length and interval on Talinum paniculatum 
(Jacq.) Gaertn adventitious roots’  biomass and saponin content in temporary immersion bioreactor. Immersion intervals 
used in this research are 3, 6, and 12 hours with immersion lengths 1, 3, 5, and 7 minutes. Adventitious roots were 
induced from leaf explants grown on MS medium added by IBA 2 mg/L. Two grams adventitious root are cultured in 
temporary immersion bioreactor with each treatment and kept for 28 days. Results of this research indicated different 
biomass and saponin content caused by each treatment. The best combination was found on immersion length of 5 
minutes and 12 hours interval which resulting 3.67 g fresh weight, growth speed 0.027 g/day, spot area of saponin  12.56 
mm2/0.01 g dry-weight, and spot area thickness scale 4+.  
Keywords: adventitious roots, saponin, temporary immersion bioreactor, Talinum paniculatum (Jacq.) Gaertn 
INTRODUCTION 
Talinum paniculatum (Jacq.) Gaertn. is a herb with 
many medicinal properties. In Indonesia T. paniculatum 
known as ginseng Jawa and it roots’  contains β-sitosteryl-
β-D-glicosida  known as pharmaceutical products 
ingredient [1]. 
T. paniculatum adventitious root induction was 
successfully done using leaf explants on MS medium 
added by growth regulator substance IBA 2 mg/L. The 
disadvantage of using solid medium is that explants only 
absorb nutrition on the lower side which have direct 
contact with the medium, so that the result is less optimal 
compared to using liquid medium [2].  
 
The use of liquid medium on plant tissue culture 
has some advantages. Liquid medium can provide 
homogenous culture condition, having faster growth rate, 
and relatively easy for sterilization [3-4]. However, there 
are several disadvantages of liquid medium mainly some 
technical issues such as hyperhydricity, cellular damage 
caused by propeller rotation when using bioreactor with 
churning system and oxygen deficiency [5]. Therefore, 
better methods to regulate aeration on the medium are 
needed. One of them is using temporary immersion 
applied to bioreactor known as Temporary Immersion 
Bioreactor (TIB) [6].  
 
TIB is bioreactor which regulates nutrition and 
oxygen absorptions of the culture. In this condition, 
explants are not immersed in the medium all the time. 
There are several periods when the explants are not 
immersed [6].  When the explants are not immersed, they 
are free to absorb oxygen because of low oxygen 
solubility on immersed condition. Oxygen is needed by 
culture to unload energy provided by medium in form of 
sucrose. TIB immersion lengths and intervals can help 
explants growth [7]. One to fifteen-minute immersion 
length with 2-12 hours of immersion frequency also 
affects explants growth of perennial plants [3].  
 
When culture is on immersed condition as a result 
of one-minute immersion length and 1, 12, 24-hour of 
immersion intervals on TIB, may cause stress on Hevea 
brasiliensis calluses identified by superoxide dismutase 
(SOD) [8]. Immersion length and interval in culture could 
cause  abiotic stresses. Abiotic stresses may affect 
production of  secondary metabolite contents [9]. 
Secondary metabolite synthesis, including saponin is a 
response to abiotic stress [10]. For example water stress 
treatment on T. paniculatum plant increase its saponin 
content on water 40% availability [11]. 
 
This research is conducted to identify the effects of 
immersion length and interval on biomass and saponin 
contents of T. paniculatum adventitious roots on liquid 
medium using temporary immersion system method on 
bioreactor. 
 
 
 


This research is conducted to identify the effects of immersion length and interval on Talinum paniculatum (Jacq.) Gaertn adventitious roots’ biomass and saponin content in temporary immersion bioreactor. Immersion intervals used in this research are 3, 6, and 12 hours with immersion lengths 1, 3, 5, and 7 minutes. Adventitious roots were

induced from leaf explants grown on MS medium added by IBA 2 mg/L. Two grams adventitious root are cultured in temporary immersion bioreactor with each treatment and kept for 28 days. Results of this research indicated different biomass and saponin content caused by each treatment. The best combination was found on immersion length of 5 minutes and 12 hours interval which resulting 3.67 g fresh weight, growth speed 0.027 g/day, spot area of saponin 12.56 mm2/0.01 g dry-weight, and spot area thickness scale 4

Yosephine Sri Wulan Manuhara, .-

Nike Oktavia Sri Saputri, .-

Alfinda Novi Kristanti, .-

Production of adventitious root and saponin of Talinum paniculatum (Jacq.) Gaertn. in temporary immersion bioreactor

http://repository.unair.ac.id/45564/13/%EF%80%A0%20-%20Bukti%20C06%20-%20Production%20of%20Adventitious%20Root%20and%20Saponin.pdf

Production of Adventitious Root and Saponin of Talinum paniculatum(Jacq.) Gaertn. in Temporary Immersion Bioreactor.

Abstract

Similar works

Full text

Available Versions

Universitas Airlangga Repository

Universitas Airlangga Repository

Universitas Airlangga Repository