Bovine hydroxyapatite and gelatin (BHA-GEL) were used as bone regeration and a biodegradable delivery system for the administration of gentamicin sulfate (GEN) in prevention and treatment infection of bone defects, were synthesized. The materials, which ovoid bone infection, are exclusively composed of gentamicin sulfate; bioactive bovine hydroxyapatite and gelatin were manufacture as pellet of the mixed components and characterized in vitro. Cross-linking reaction was required to control the water penetration, swelling and release of gentamicin from the pellet. In vitro gentamicin release from the pellet at conditions of pH and temperature body were studied for 4 weeks and the sample was able to inhibit of Staphylococcus aureus and Escherichia coli growth, both in surrounding liquid and on matrix surface. The BHA-GEL-GEN crosslinking with glutaraldehyde (GA) were nontoxic to human osteoblasts and promote their proliveration. The results indicate that BHA-GEL-GEN with GA non-toxic and selfiendly-is promising biomaterial of significantly prolonged antibacterial activity
