A simple and fast HPLC method has been validated for the determination of clotrimazole in nanocapsule suspensions prepared from an acrylic polymer, Eudragit RS100®. Chromatographic runs were performed on a RP C18 column with a mobile phase comprising methanol and water (90:10, v/v) at a flow rate of 1.0 mL/min with UV detection at 229 nm. The method was shown to be specific, linear (r = 0.9987), precise and accurate in a concentration range of 2.0-10.0 μg/mL. The proposed method can be successfully used to quantify clotrimazole content in acrylic nanocapsule formulations, as well as to estimate drug encapsulation efficiency.Colegio de Farmacéuticos de la Provincia de Buenos Aire
