Aslanidou, Lydia

Ferraro, Mauro

Logghe, Gerlinde

Lovric, Goran

Segers, Patrick

Stergiopulos, Nikolaos

Trachet, Bram

English

Ghent University Academic Bibliography

J25th Congress of theEuropeen Society of Biomechanics1..............ÏLM.LUParallel Sessions10:1510:3010:4511:0011:1511:3011:45TR06.6Cardiovascular biomechanicsChair: D. Gallo, E. PenaBIG HSTR07.2Cellular and sub-cellular biomechanicsChair: GJ. Schütz, N.D. EvansHS 03TR08.1Clinical biomechanics and computer-aided medical interventionsChair: M.M. Morlock, L. CristofoliniSR 06LAYER-SPECIFIC DAMAGE PROPERTIESOF THORACIC AND ABDOMINAL PORCINE AORTA: EXPERIMENTS AND CONSTITUTIVEMODELINGJ.A. Pena. M.A. Martinez. E. PenaCOMPUTER SIMULATIONS OF ELECTROME- CHANICAL DRUG EFFECTS ON HUMANSTEM CELL-DERIVED CARDIAC TISSUEA. June. M. GoBmann. P. Linder. M. StaatINCORPORING SUBJECT-SPECIFIC PARAME­TERS IN GENERIC COMPUTATIONAL MOD­ELS FOR ASSESSING CLINICAL OUTCOMESP. PankaiSYNCHROTRON-BASED PRESSURE IN- FLATION EXPERIMENTS TO MODEL THE MOUSE CAROTID ARTERY MICROSTRUC-TURE IN 3DB.Trachet. M.Ferraro. G. Lovric. L. Aslanidou.G. Logghe, N. Stergiopulos, P. SegersCANCER CELL MIGRATION IN CONFINED ENVIRONMENTSI. Badia-Villacampa, Y. Juste-Lanas,J. M. Garcia-Aznar, M J. Gomez-BenitoBIOMECHANICA!. EX-VIVO RESPONSE OF ARTERIES SUBJECTED TO HYPOXIA CONDI-TIONSA. Navarrete, C. Garcia-Herrera, E. Herrera,B. Krause, A. Gonzalez-Candia, D. Celentano,E. RiveraIN-SITU ANALYSIS OF TIME-DEPENDENT FORMATION ON CARTILAGE COLLAGEN STRUCTURE UNDER MECHANICAL STIMU- LATIONK. Hamada, Y. Morita. E. Nakamachi.K. Yamamoto3D MOTION ANALYSIS OF THE WRISTDURING DART-THROWING MOTION AFTERPARTIAL WRIST FUSIONG. Fischer, L. Reissner. M. CalcagniLOCAL IN VITRO EVALUATION OF THE BIOMECHANICAL PROPERTIES OF THE ASCENDING AORTIC ANEURYSMSS. Lin. M. Morgan, A. Lalande, A. Cochet,O. BouchotANALYSIS OF SURFACE ROUGHNESS INFLU- ENCE DURING COLLECTIVE CELL MIGRA­TIONT. Thenard, M.Mesnard, A. Cataoano.R. AllenaCOMPUTATION OF SUBTALAR JOINT CEN-TRE OF ROTATION IN WEIGHT-BEARINGCLINICAL CT USING DVCM. Pena Fernandez, D. Hoxha. G.W. Blunn. G.Tozzi, A.GoldbergCOMPUTATIONAL EVALUATION OF LONG­TERM IN-STENT RESTENOSIS BASED ON TISSUE GROWTH MODELR. He. L. Zhao, V.V. Silberschmidt, Y. Liu .INVESTIGATING THE STRUCTURAL AND MECHANICAL PROPERTIES UNDERLYINGMELANOMA TRANSITIONG. Higeins. T. Abdalrahman, M.H.Zaman,J. Peres, S. Prince, T. FranzIN SILICO MODEL-BASED PATIENT-SPECIFIC SOLUTION SYSTEM FOR BLEPHAROPTOSISSURGERYR. Huang. R. Oeden. R. Penta. 1. Cordon.S. Drummond, W. IonTHE EFFECT OF INFLAMED ENDOTHELIAL CELL ON SHEAR-ACTIVATED PLATELETS IN ENDOVENTRICULAR THROMBOSISF. Consolo, N. Bono, A. Dimasi, K.R.Ammann,Y. Roka Moiia, G.B. Fiore, F. Pappalardo,G. Candiani, M.J.SIepian, A. RedaelliLOCAL MEMBRANE STRESSES ANDSTRAINS IN AN ERYTHROCYTE DURING INVASION BY A MALARIA MEROZOITEC. Msosa. T. Abdalrahman. T. FranzSUBJECT-SPECIFIC HEAD MODEL DEVELOP- MENT FROM A PORTABLE CONTACTLESS SENSOR FOR FACIAL MIMIC ANALYSIS AND MODELINGT. Nguven, S. Dakoé. M. Ho Ba Tho. T.T. DaoSENSITIVITY ANALYSIS OF A THROMBEC- TOMY FINITE-ELEMENT MODEL THROUGHA KRIGING PROCESSG. Luraehi. S. Pant. F. Mieliavacca.J.F. Rodriguez MatasEXPERIMENTAL MECHANICS OF INDIVID- UAL COLLAGEN MOLECULESA. Rohatschek. O.G. Andriotis. P. Steinbauer.S. Baudis, C. Dworak, PJ.ThurnerIN VIVO MOTION ARTIFACTS SIGNIFI-CANTLY DEGRADE IMAGE REGISTRATIONACCURACYP.R. Atkins. K.Simon. L. Horling. N. Ohs.P. Christen, M. Blauth, R. Müller50 25th Congress of the European Society of Biomechanics, July 7-10, 2019, Vienna, Austria SYNCHROTRON-BASED PRESSURE INFLATION EXPERIMENTS TO MODEL THE MOUSE CAROTID ARTERY MICROSTRUCTURE IN 3D Bram Trachet (1,2), Mauro Ferraro (2), Goran Lovric (3), Lydia Aslanidou (2), Gerlinde Logghe (1), Nikolaos Stergiopulos (2), Patrick Segers (1)  1. IBiTech-bioMMeda, Ghent University, Belgium; 2. Institute for Bioengineering, EPFL, Switzerland; 3. Swiss Light Source, Paul Scherrer Institute, Villigen, Switzerland.  Introduction The contribution of the aortic micro-structure to mechanics is still poorly understood [1]. Multiphoton imaging (MPM) has recently been used to visualize how lamellae in the mouse carotid artery react to increasing pressure [2]. However, MPM has a limited field of view and is restricted to a segment of the arterial wall. Synchrotron-based imaging could offer an alternative, but is typically used for non-pressurized, ex vivo samples. In order to overcome that limitation, we developed a synchrotron-compatible pressure inflation device that allows for quasi-static imaging of the mouse carotid artery at different pressure levels.   Methods Six wild type (WT) and six ApoE-/- mice, all male and on a C57Bl6/J background, were used for this study. After excising the left carotid artery it was mounted on our in-house developed device. Intra-luminal pressure was increased quasi-statically from 0 to 120 mmHg with a syringe pump. At each pressure level synchrotron-based phase-propagation imaging was performed at 25m source-to-sample distance, at 25 cm sample-to-detector distance and at 21 keV. The effective voxel size was 1.625 µm3. During the scans the axial stretch was kept at the in vivo value. For visualization purposes we skipped 2 out of every 3 axial images in the analysis, reducing the effective axial resolution to 4.9 µm and resulting in a stack of 75 images per pressure level (representing an axial length of 366 µm). The total dataset thus consisted of 8100 images (12 animals x 9 pressure levels x 75 images). Images were segmented using an in-house developed automated segmentation algorithm. Arterial diameter, length, thickness and straightness were quantified, where straightness was defined as the ratio of (i) the true lamellar length and (ii) the length of a least-square fitted circle through the lamellar data points.  Results All three lamellar layers straightened and stretched simultaneously when intraluminal pressure was increased, confirming earlier reports [2]. In the inner lamella, lamellar length increased from 0.97 mm to 1.57 mm while the corresponding luminal diameter increased from 0.28 to 0.48 mm. This resulted in an increase in straightness from 0.89 to 0.97. The steepest increase in lamellar straightness occurred between 0 and 30 mmHg, while the lamellar length increased quasi-linearly over the entire pressure range. We did not find any statistically significant difference between WT and ApoE -/- mice.  Finally, 3D models were created for each pressure level. Three lamellae and two interlamellar layers could be segmented independently. The adventitia was modelled as a homogeneous layer.  Conclusions We present a novel methodology to visualize and segment arterial lamellae and tunica adventitia along the entire circumference of the mouse carotid artery. In a next step, the 3D models we created will form the input for computational biomechanics of the arterial micro-structure. Ultimately, we hope that such models will contribute to a better understanding of the overall mechanics of the arterial wall.  References 1.Trachet B, Aslanidou L, Piersigilli A, Fraga-Silva AR, Sordet-Dessimoz J, Villanueva-Perez P, Stampanoni M, Stergiopulos N, Segers P. Angiotensin II infusion into ApoE-/- mice: a model for aortic dissection rather than  abdominal aortic aneurysm? Cardiovasc. Res., 113: 1230-42, 2017.  2. Yu X, Turcotte R, Seta F, Zhang Y. Micromechanics of elastic lamellae: unravelling the role of structural inhomogeneity in multi-scale arterial mechanics. J. R. Soc. Interface, 15: 20180492, 2018. Figure 1: (a) Synchrotron images and (b) corresponding 3D segmentations of arterial lamellae and tunica adventitia in the mouse left carotid artery. 

Synchrotron-based pressure inflation experiments to model the mouse carotid artery microstructure in 3D

https://biblio.ugent.be/publication/8623311/file/8623312.pdf

Synchrotron-based pressure inflation experiments to model the mouse carotid artery microstructure in 3D

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