This research was preliminary study of the presence and prevalence of Chlamydophila felis (Cp. felis) among domestic cats in the City of Zagreb and Zagreb County in Croatia. 48 pure and mixed breed cats were examined. 192 conjunctival and nasal swab samples were examined using a rapid immunoenzyme assay (EIA). 30 conjunctival scraping samples of 15 cats that were EIA positive were examined using DIF. Four of them were positive, while the results of two samples were inconclusive. These six samples (three nostril samples and three eye samples) were examined using a conventional polymerase chain reaction (PCR), and only two samples from the same cat were found to be positive for Cp. felis. The sequencing of the PCR product confirmed that the causative agent of the cat’s conjunctivitis was Cp. felis. By using direct immunofluorescence as well as a polymerase chain reaction procedure and sequencing, the results of the research established the existence of the bacteria Cp. felis in one out of 48 domestic cats.Cilj ovog preliminarnog istraživanja bio je ustanoviti učestalost pojave bakterije Chlamydophila felis (Cp. felis) u domaće mačke u Gradu Zagrebu i na području Zagrebačke županije. Istraživanjem je bilo obuhvaćeno 48 mačaka različitih pasmina. Brzim enzimskim imunološkim dijagnostičkim postupkom (EIA) pretraženo je 192 uzoraka obrisaka konjunktiva i nosnica te 12 uzoraka obriska sluznice rektuma. Trideset obrisaka konjuktiva s 15 mačaka, pozitivnih na EIA, pretraženo je postupkom izravne imunofluorescencije (DIF). Četiri su uzorka bila je pozitivna, a u dva je nalaz bio sumnjiv. Potom je tih šest uzoraka (tri uzoraka obriska nosne sluznice i tri
obriska konjunktive) pretraženo postupkom lančane reakcije polimerazom (PCR). Dva su uzorka, podrijetlom s iste mačke, bila pozitivna. Rezultat sekvencioniranja produkta PCR dokazao je da je bolest u te domaće mačke bila uzrokovana upravo bakterijom Cp. felis. Korištenjem postupka izravne imunofluorescencije, lančane reacije polimerazom te sekvenciranjem produkta PCR dokazano je postojanje bakterije Cp. felis kao uzročnika mačje klamidioze u jedne od 48 mačaka
