A. Kicheva

Alexander Bläßle

AP Pomreinke

C Nüsslein-Volhard

Charles B. Kimmel

DM Umulis

ELIZABETH M. DEUCHAR

J Schindelin

J Zinski

KW Rogers

L. Wolpert

M Westerfield

MM Poo

N Lorén

P Müller

P. A. Liebman

Q Xu

S Harmansa

Thomas Gregor

Morphogens are signaling molecules that provide positional information to cells during development. They must move through embryonic tissues in order to coordinate patterning. The rate of a morphogen's movement through a tissue-its effective diffusivity-affects the morphogen's distribution and therefore influences patterning. Fluorescence recovery after photobleaching (FRAP) is a powerful method to measure the effective diffusion of molecules through cells and tissues, and has been successfully employed to examine morphogen mobility and gain important insights into embryogenesis. Here, we provide detailed protocols for FRAP assays in vitro and in living zebrafish embryos, and we explain how to analyze FRAP data using the open-source software PyFRAP to determine effective diffusion coefficients

FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos

Abstract

Similar works

Full text

Available Versions

MPG.PuRe

Crossref