Bakterijska adhezija na površinah in pozneje nastanek biofilma predstavljata glavni
vzrok kroničnih okužb z negativnim vplivom na zdravje ljudi. S tem problemom se
srečujemo tudi pri izbiri in izdelavi pohištva, kjer stremimo k razvoju izdelkov s
takšnimi površinami, na katere naj se bakterije ali drugi mikroorganizmi praviloma
ne bi oprijemali. Predmet naše raziskave je bil testirati izbrana premazna sredstva za
les (laneno olje, vodna lazura, nitrocelulozni lak, nanopremaz) na adhezijo bakterij
vrste Pseudomonas aeruginosa, kjer smo kot testno površino uporabili vezano
bukovo ploščo. Topografijo testne površine in število oprijetih bakterij P.
aeruginosa smo opazovali s SEM. Na podlagi kvantitativne analize SEM slik smo
določili število oprijetih bakterij z obkroževanjem le-teh. Preliminarne rezultate smo
predstavili v obliki posnetkov SEM. Rezultati nakazujejo, da se je največje število
bakterij oprijelo na površini, premazani z lanenim oljem. Ugotovili smo, da lahko s
SEM mikroskopom ob ustrezni metodologiji dela opazujemo adhezijo bakterij na
lesenih površinah. Za potrditev in pridobitev relevantnih rezultatov bodo v
prihodnosti potrebne obširnejše in bolj poglobljene študije.Bacterial adhesion to surfaces and subsequent biofilm formation are the main causes
of chronic infections with a negative impact to the human health, and this is a
problem in the process of manufacturing furniture. We this aimed to develop
antibacterial surfaces to which the microorganisms do not adhere. The focus of our
research was to test selected wood coatings (linseed oil, water-borne stain,
nitrocellulose varnish, nano-coating) with regard to the adhesion of Pseudomonas
aeruginosa, where we used a beech plywood as the test surface. The topography of
the test surface and number of adhering bacteria were observed on SEM
micrographs. The number of adhering bacteria was determined using quantitative
analysis of the SEM images. Preliminary results were presented in the form of SEM
recordings. The results indicate that the maximum number of bacteria adhered to the
surface coated with linseed oil. We found that by using an SEM microscope, and the
appropriate methodology of work, we can observe the adhesion of bacteria on
wooden surfaces. In the future, extensive and in-depth studies will be needed to
confirm and obtain the relevant results
