New fast liquid chromatographic and capillary zone electrophoresis methods were developed and validated for simultaneous determination of atenolol and chlortalidone in combined dose tablets. The reversed phase HPLC method was carried out on a CN LiChrosorb® (125 x 4 mm, 5 μm) column. The CZE method was carried out on an uncoated fused-silica capillary of 30 cm x 75 μm i.d. with 25 mmol L-1 sodium tetraborate, pH 9.4. The total analysis time was <6 and <2.5 min for HPLC and CZE methods, respectively. Both methods can be used for stability studies as well.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Almeida, Mariana M. de

Alves, João M. P.

Hackmann, Erika R. M. K.

Leite, Heitor O.A.

Lima, Cibele R. R. C.

Patto, Daniela C.S.

Prado, Maria S. A.

Santoro, Maria Ines Rocha Miritello

Singh, Anil K.

Tavares, Vanessa F.

New fast liquid chromatographic and capillary zone electrophoresis methods were developed and validated for simultaneous determination of atenolol and chlortalidone in combined dose tablets. The reversed phase HPLC method was carried out on a CN LiChrosorb (R) (125 x 4 mm, 5 mu m) column. The CZE method was carried out on an uncoated fused-silica capillary of 30 cm x 75 mu m i.d. with 25 mmol L(-1) sodium tetraborate, pH 9.4. The total analysis time was <6 and <2.5 min for HPLC and CZE methods, respectively. Both methods can be used for stability studies as well

PATTO, Daniela C. S.

LEITE, Heitor O. A.

TAVARES, Vanessa F.

ALMEIDA, Mariana M.

LIMA, Cibele R. R. C.

ALVES, Joao M. P.

PRADO, Maria S. A.

HACKMANN, Erika R. M. K.

SANTORO, Maria Ines R. M.

SINGH, Anil K.

RCAAP - Repositório Científico de Acesso Aberto de Portugal

High Throughput Simultaneous Assay of Atenolol and Chlortalidone in Combined Dose Tablets by Liquid Chromatography and Capillary Zone Electrophoresis

SEDICI - Repositorio de la UNLP

594 ISSN 0326-2383
KEY WORDS: Atenolol, Capillary zone electrophoresis, Chlortalidone, Liquid chromatography Quantitative de-
termination.
* Author to whom correspondence should be addressed. E-mail: anil@usp.br
Latin American Journal of Pharmacy
(formerly Acta Farmacéutica Bonaerense)
Lat. Am. J. Pharm. 29 (4): 594-601 (2010)
Original Article
Received: August 25,  2009
Accepted: November 20, 2009
High Throughput Simultaneous Assay of Atenolol
and Chlortalidone in Combined Dose Tablets
by Liquid Chromatography and Capillary Zone Electrophoresis
Daniela C.S. PATTO, Heitor O.A. LEITE, Vanessa F. TAVARES, Mariana M. ALMEIDA,
Cibele R. R. C. LIMA, João M. P. ALVES, Maria S. A. PRADO,
Erika R. M. K. HACKMANN, Maria Inês R. M. SANTORO & Anil K. SINGH*
Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo,
CP 66083, CEP 05315-970, São Paulo, SP, Brazil.
SUMMARY. New fast liquid chromatographic and capillary zone electrophoresis methods were developed
and validated for simultaneous determination of atenolol and chlortalidone in combined dose tablets. The
reversed phase HPLC method was carried out on a CN LiChrosorb® (125 x 4 mm, 5 μm) column. The
CZE method was carried out on an uncoated fused-silica capillary of 30 cm x 75 μm i.d. with 25 mmol L–1
sodium tetraborate, pH 9.4. The total analysis time was <6 and <2.5 min for HPLC and CZE methods, re-
spectively. Both methods can be used for stability studies as well.


English

High throughput simultaneous assay of atenolol and chlortalidone in combined dose tablets by liquid chromatography and capillary zone electrophoresis

New fast liquid chromatographic and capillary zone electrophoresis methods were developed and validated for simultaneous determination of atenolol and chlortalidone in combined dose tablets. The reversed phase HPLC method was carried out on a CN LiChrosorb (R) (125 x 4 mm, 5 mu m) column. The CZE method was carried out on an uncoated fused-silica capillary of 30 cm x 75 mu m i.d. with 25 mmol L(-1) sodium tetraborate, pH 9.4. The total analysis time was <6 and <2.5 min for HPLC and CZE methods, respectively. Both methods can be used for stability studies as well.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq

Universidade de São Paulo

El Servicio de Difusión de la Creación Intelectual

Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)

Servicio de Difusión de la Creación Intelectual

594 ISSN 0326-2383KEY WORDS: Atenolol, Capillary zone electrophoresis, Chlortalidone, Liquid chromatography Quantitative de-termination.* Author to whom correspondence should be addressed. E-mail: anil@usp.brLatin American Journal of Pharmacy(formerly Acta Farmacéutica Bonaerense)Lat. Am. J. Pharm. 29 (4): 594-601 (2010)Original ArticleReceived: August 25,  2009Accepted: November 20, 2009High Throughput Simultaneous Assay of Atenololand Chlortalidone in Combined Dose Tabletsby Liquid Chromatography and Capillary Zone ElectrophoresisDaniela C.S. PATTO, Heitor O.A. LEITE, Vanessa F. TAVARES, Mariana M. ALMEIDA,Cibele R. R. C. LIMA, João M. P. ALVES, Maria S. A. PRADO,Erika R. M. K. HACKMANN, Maria Inês R. M. SANTORO & Anil K. SINGH*Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo,CP 66083, CEP 05315-970, São Paulo, SP, Brazil.SUMMARY. New fast liquid chromatographic and capillary zone electrophoresis methods were developedand validated for simultaneous determination of atenolol and chlortalidone in combined dose tablets. Thereversed phase HPLC method was carried out on a CN LiChrosorb® (125 x 4 mm, 5 μm) column. TheCZE method was carried out on an uncoated fused-silica capillary of 30 cm x 75 μm i.d. with 25 mmol L–1sodium tetraborate, pH 9.4. The total analysis time was <6 and <2.5 min for HPLC and CZE methods, re-spectively. Both methods can be used for stability studies as well.

http://sedici.unlp.edu.ar/bitstream/handle/10915/7953/Resumen.pdf?sequence=1

High throughput simultaneous assay of atenolol and chlortalidone in combined dose tablets by liquid chromatography and capillary zone electrophoresis

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