he Ames Test approach. However the cell permeability of yeast has been considered a limitant
factor to this kind of assay and many researchers have been introducing genetic modifications into
wild strains to improve the sensitivity to chemical compounds. In our study, we used Saccharomyces cerevisiae
ATCC 9763, well known and very common strain in antibiotic assays, and we evaluated the cytotoxicity
of some antineoplastic agents (etoposide, epirubicin, carboplatin, cisplatin and mitoxantrone). Each
culture was observed under the light of microscope and photographed. Neither genetic modification nor
addition of permeation inducers, as dimethylsulfoxide (DMSO), were introduced during the assays and the
cells presented good sensitivity to those compounds, demonstrating that other potential strains and characteristics
of cells should be reconsidered to improve these assays apart from the cellular permeability.Colegio de Farmacéuticos de la Provincia de Buenos Aire