Contains fulltext :
                  208073.pdf (publisher's version ) (Open Access

Abdollahi-Roodsaz, Shahla

Afd Pharmacology

Boekhorst, Jos

Ederveen, Thomas H A

Garssen, Johan

Hartog, Anita

Helsen, Monique M

Knol, Jan

Koenders, Marije I

Pharmacology

Rogier, Rebecca

Sub Theoretical Biology & Bioinformatics

van de Loo, Fons A J

van der Kraan, Peter M

van Hijum, Sacha A F T

van Lent, Peter L E M

Walgreen, Birgitte

Wopereis, Harm

Texto & Contexto Enfermagem

Rogier, R.L.

Ederveen, T.H.

Wopereis, H.

Boekhorst, J.

Hijum, S.A.F.T. van

Walgreen, B.

Helsen, M.M.A.

Kraan, P.M. van der

Lent, P.L.E.M. van

Loo, F.A.J. van de

Abdollahi-Roodsaz, S.

Koenders, M.I.

NARCIS 

Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice

The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis. Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome. This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis. Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota. The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice. We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota. Interestingly, the genera Enterococcus and Clostridium were markedly decreased by scGOS/lcFOS dietary supplementation. In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet. However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice. As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks. Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice

Utrecht University Repository

Rebecca Rogier

Thomas H A Ederveen

Harm Wopereis

Anita Hartog

Jos Boekhorst

Sacha A F T van Hijum

Jan Knol

Johan Garssen

Birgitte Walgreen

Monique M Helsen

Peter M van der Kraan

Peter L E M van Lent

Fons A J van de Loo

Shahla Abdollahi-Roodsaz

Marije I Koenders

Directory of Open Access Journals

PLoS ONE

Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.

Radboud Repository

The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis. Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome. This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis. Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota. The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice. We here show that dietary supplementation with scGOS/ lcFOS has a pronounced effect on the composition of the fecal microbiota. Interestingly, the genera Enterococcus and Clostridium were markedly decreased by scGOS/lcFOS dietary supplementation. In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet. However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice. As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks. Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice

Ederveen, Thomas H.A.

Van Hijum, Sacha A.F.T.

Helsen, Monique M.

Van Der Kraan, Peter M.

Van Lent, Peter L.E.M.

Van De Loo, Fons A.J.

Koenders, Marije I.

The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis. Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome. This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis. Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota. The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice. We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota. Interestingly, the genera Enterococcus and Clostridium were markedly decreased by scGOS/lcFOS dietary supplementation. In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet. However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice. As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks. Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.</div

Rebecca Rogier (4171054)

Thomas H. A. Ederveen (411978)

Harm Wopereis (175116)

Anita Hartog (3558266)

Jos Boekhorst (81341)

Sacha A. F. T. van Hijum (9843)

Jan Knol (175123)

Johan Garssen (278720)

Birgitte Walgreen (3716071)

Monique M. Helsen (3716077)

Peter M. van der Kraan (714850)

Peter L. E. M. van Lent (714849)

Fons A. J. van de Loo (285352)

Shahla Abdollahi-Roodsaz (531410)

Marije I. Koenders (158627)

The Francis Crick Institute

Helena Maria Scherlowski Leal David

Maria Yvone Chaves Mauro

Viviane Gomes Silva

Michely Alexandrino de Souza Pinheiro

Fernanda Henriques da Silva

Vieira ALS

Robazzi MLCC

Levcovitz E

Ribeiro EM

Carvão JM

Dejours C

Franco T

Mauro MYC

Pedrosa JIS

Oliveira EM

Matumoto S

Almeida CMD

Teixeira CP

Cattani AD

Murofuse NT

Marziale MHP

David HMSL

Pereira MCA

Crossref