Huang, Wei

Tang, Jing

Werchmeister, Rebecka Maria Larsen

Wollenberger, Ulla

Zhang, Jingdong

Online Research Database In Technology

  General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.   Users may download and print one copy of any publication from the public portal for the purpose of private study or research.  You may not further distribute the material or use it for any profit-making activity or commercial gain  You may freely distribute the URL identifying the publication in the public portal  If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.         Downloaded from orbit.dtu.dk on: Sep 11, 2019Graphene-Sulfite Oxidase Bioanodes for Enzymatic Biofuel CellsTang, Jing; Werchmeister, Rebecka Maria Larsen; Huang, Wei; Wollenberger, Ulla; Zhang, JingdongPublication date:2019Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Tang, J., Werchmeister, R. M. L., Huang, W., Wollenberger, U., & Zhang, J. (2019). Graphene-Sulfite OxidaseBioanodes for Enzymatic Biofuel Cells. Poster session presented at The Danish Chemical Society AnnualMeeting 2019, Copenhagen, Denmark.Ændre størrelse på overskriften Klik på ‘Formindsk indryk’ Ændre størrelse på Manchet, Mellemrubrik og Brødtekst Klik en gang på  ‘Forøge indryk‘ for at få mindre Manchet Klik 2.gang på ‘Forøge indryk‘ for at få Fed Klik 3.gang på ‘Forøge indryk‘ for at få Regular Klik 4.gang på ‘Forøge indryk‘ for at få Fed Klik 5.gang på ‘Forøge indryk‘ for at få Regular Klik på ‘Formindsk Graphene-sulfite oxidase bioanodes for enzymatic biofuel cellsJ. Tang1, R. M. L. Werchmeister1, U. Wollenberger2, C. Engelbrekt1, J. Zhang1*IntroductionEnzymatic biofuel cells (EBFCs), using enzymes as catalysts on theanode or/and cathode, offer a wide range of abundant biological fuelssuch as starch, glucose, fructose and lactate, etc.Sch. 1 an EBFC with a mediatedbioanode and a direct electron transfer-based biocathode.Fig. 5 Polarization and power density curves of EBFCs and control FCs with CP-GO_RGO-PEI_R as the anode at 30 °C.1 Technical University of Denmark, Department of Chemistry, Kemitorvet 207, 2800 Kgs. Lyngby, Denmark, jz@kemi.dtu.dk2 University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht Strasse 24-25, 14476 Golm, GermanyPreparation of bioanodes and electrochemical characterizationEvaluation  of sulfite/O2 EBFCsFig. 2 Cyclic voltammetry of thebioanode in the absence (dash)and presence (solid) of sulfite in750 mM Tris-acetate buffer, pH8.4, 5 mV s-1.The EBFCs with an OCV of 0.64 ± 0.01 V reach a maximum powerdensity (Pmax, 61 ± 6 µW cm-2), while control FCs show an OCV of 0.40 ±0.06 V and a Pmax of 4 ± 2 µW cm-2. It indicates that the sulfite/O2 EBFCsworks.PerspectivesAcknowledgmentsFinancial support from Danish Council for Independent ResearchTechnology and Production Sciences (DFF –YDUN 4093-00297) isgreatly acknowledged.Fabrication a biocathode for EBFCs will be the next step.NanoChemistrySch. 2 Fabrication of a CP-GO_RGO-PEI_R_HSO bioanode. CP: carbonpaper; GO: graphene oxide; RGO: reduced graphene oxide; PEI:polyethylenimine; HSO: human sulfite oxidase.Sulfite oxidases (SO) catalyze theoxidation of 𝑆𝑂32− to 𝑆𝑂42−. Thus, they canbe employed to fabricate sulfite/O2 EBFCsusing sulfite as the energy source.0 100 200 3000.20.40.6 EBFCs Control FCs EBFCs Control FCsCurrent density (mA cm-2)Voltage (V)020406080 Power density (mW cm-2)The reduced bioanode exhibits both 10-fold catalytic and capacitivecurrent of the non-reduced one, Fig. 2. The promoted catalytic andelectrochemical properties after reducing GO on electrodes, are due tothe increased conductivity of RGO compared to GO, and the electriccontact points between electrode surface and SO.Fig. 1 Structure of the sulfite oxidase, with the mobile heme domain in orange, and the metallic cofactors (molybdenum active site and heme) shown as sticks.J. Am. Chem. Soc. 2017, 139, 11559−11567.Although most EBFCs use organic and biological molecules as fuels, inorganics can also be utilized for EBFCs with suitable enzymes.Anode: 𝑆𝑂32− + 𝐻2𝑂 → 𝑆𝑂42− + 2𝐻+ + 2𝑒−Cathode:12𝑂2 + 2𝐻+ + 2𝑒− → 𝐻2𝑂-0.9 -0.6 -0.3 0.0-1.2-0.9-0.6-0.30.0 Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Cycle 6 Cycle 7 Cycle 8 Cycle 9 Cycle 10Current (mA)Potential (V vs. Ag/AgCl)Fig. 3 Electrochemical reduction.Fig. 4 Digital photo and scheme of assembled EBFCs.

Graphene-Sulfite Oxidase Bioanodes for Enzymatic Biofuel Cells

https://orbit.dtu.dk/ws/files/189007830/Poster.pdf

Graphene-Sulfite Oxidase Bioanodes for Enzymatic Biofuel Cells

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology