Microbiological preparation of 3H‐labelled methane

Abstract

Small quantities of tritiated methane were prepared by fermenting [methyl‐3H] methylamine hydrochloride using exponential phase, transiently starved, co‐cultures of methanogenic bacteria. This method gave the expected 75% yield of C3H4 from the substrate and produced H2‐free, low 3H2O background C3H4 for tracer studies. Copyright © 1989 John Wiley & Sons, Ltd