Fantini, S

Franceschini, MA

Gratton, E

Hueber, D

Maulik, D

Rosenfeld, W

Stankovic, MR

Stubblefield, PG

Stankovic, Miljan R

Maulik, Dev

Rosenfeld, Warren

Stubblefield, Phillip G

Franceschini, Maria Angela

Hueber, Dennis

Gratton, Enrico

Fantini, Sergio

eScholarship - University of California

UC IrvineUC Irvine Previously Published WorksTitleFirst RealTime Optical Imaging of Experimental Brain Ischemia and Hemorrhage in Neonatal PigletsPermalinkhttps://escholarship.org/uc/item/65m2q6fvJournalPediatric Research, 45(7)ISSN0031-3998AuthorsStankovic, Miljan RMaulik, DevRosenfeld, Warrenet al.Publication Date1999-04-01DOI10.1203/00006450-199904020-02066Copyright InformationThis work is made available under the terms of a Creative Commons Attribution License, availalbe at https://creativecommons.org/licenses/by/4.0/ Peer reviewedeScholarship.org Powered by the California Digital LibraryUniversity of CaliforniaThe American Pediatric Society and The Society for PediatricResearch 1999 AbstractPediatric Research (1999) 45, 347A–347A; doi:10.1203/00006450-199904020-02066First RealTime Optical Imaging of Experimental Brain Ischemia andHemorrhage in Neonatal PigletsMiljan R Stankovic1, Dev Maulik1, Warren Rosenfeld1, Phillip G Stubblefield1, MariaAngela Franceschini1, Dennis Hueber1, Enrico Gratton1 and Sergio Fantini11Winthrop Univ. Hospital, Mineola, NY, Boston Univ., Boston, MA, Dept Physics, Urbana, IL, ISS, Champaign, ILAbstract 2050 Poster Session II, Sunday, 5/2 (poster 229)Optical imaging, based on the ability of near infrared light to non-invasively penetrate through the intactscalp and skull, can monitor cerebral hemodynamics and oxygenation in real-time, assuming thatimages are acquired fast enough. Previously reported acquisition times of 5 s, 10 min, 2 h, or even 1 to 3days per image, were far too slow to allow for real-time imaging, particularly in clinical settings whereacute events are the focus of our attention. This study presents the first application of real-time opticalimaging in the detection of experimental brain ischemia and hemorrhage and the accompanyingcerebrovascular changes in newborn piglets. Optical imaging was performed on 7 anesthetized,instrumented, and ventilated newborn piglets secured within a stereotaxic instrument. We used amodified near-infrared oximeter and a custom-built 16 - light - source - 2 - detector optical proberendering 64 pixel / 4 × 4 cm / 2-D brain absorption images with the acquisition time of 0.19 s perimage (or 5.26 images per sec). Once the baseline was recorded (Fig 1), 0.3 cc of saline and 2 cc ofblood were injected into the left frontal subcortical brain region aimed to induced focal and globalanatomical and functional brain absorption changes. The imager readily detected: 1) focal changes inbrain absorption caused by the appearance of the saline (white changes) and blood (dark changes) in thebrain featuring brain ischemia and hemorrhage, respectively; 2) global bilateral ischemic changes(white changes) caused by the accompanying increase in intracranial pressure featuring brain ischemiafollowed by reperfusion and hyperemia; 3) five subcortical hematomas and two large bilateralsubarachnoid hemorrhages with small subcortical hematomas, as confirmed on autopsy. The imagingdetection limit was ~0.07 cc (5 mm in diameter) for saline and ~0.04 cc (4 mm in diameter) for blood atthe estimated depth of 1 -1.5 cm. Our 1-min movie clip demonstrates the capability of optical imaging indetecting brain ischemia, hemorrhage, and reperfusion non-invasively and real-time, with hightemporal and good spatial resolution.Fig 1.No captionFull figure and legend (64K)

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First RealTime Optical Imaging of Experimental Brain Ischemia and Hemorrhage in Neonatal Piglets

First RealTime optical imaging of experimental brain ischemia and hemorrhage in neonatal piglets

First RealTime optical imaging of experimental brain ischemia and hemorrhage in neonatal piglets

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