Sanna Vuoristo, Ismo Virtanen, Minna Takkunen, Jaan Palgi, Yamato Kikkawa, Patricia Rousselle, Kiyotoshi Sekiguchi, Timo Tuuri, and Timo Otonkoski, "Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support", Journal of Cellular and Molecular Medicine, 13, 8b, pp. 2622-2633, Wiley, 200

Bendall

Bloor

Braam

Cheresh

Conover

Cooper

da Silva Meirelles

Damsky

Domogatskaya

Dubey

Durbeej

Ekblom

Engvall

Fradet

Fujiwara

Giltay

Ginis

Gustafsson

Hemler

Ismo Virtanen

Jaan Palgi

Kalluri

Kikkawa

Kiyotoshi Sekiguchi

Kortesmaa

Leivo

Lensch

Ludwig

Maatta

Mikkola

Miner

Minna Takkunen

Miyazaki

Pasqualini

Patricia Rousselle

Petajaniemi

Prater

Rahuel

Rousselle

Sanna Vuoristo

Scadden

Scheele

Smyth

Solter

Takkunen

Tamura

Tanentzapf

Timo Otonkoski

Timo Tuuri

Ullmann

Vainionpaa

Van Hoof

Virtanen

Wayner

Werb

Wewer

Wondimu

Yamada

Yamato Kikkawa

Ylanne

English

Crossref

Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support

Vuoristo, Sanna

Virtanen, Ismo

Takkunen, Minna

Palgi, Jaan

Kikkawa, Yamato

Rousselle, Patricia

Sekiguchi, Kiyotoshi

Tuuri, Timo

Otonkoski, Timo

Osaka University Knowledge Archive

Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support

To reveal the functional intrinsic niche of human embryonic stem cells (hESC) we examined the production of basement membrane (BM) proteins and the presence of their receptors in feeder‐free cell culture conditions. In addition, we investigated binding of hESCs to purified human BM proteins and identified the receptors mediating these contacts. Also, we tested whether purified human laminin (Lm) isoforms have a role in hESC self‐renewal and growth in short‐term cultures. The results show that hESCs synthesize Lm α1 and Lm α5 chains together with Lm β1 and γ1 chains suggesting the production of Lms‐111 and ‐511 into the culture medium and deposits on cells. hESCs contain functionally important integrin (Int) subunits, Int β1, α3, α6, α5, β5 and αV, as well as the Lm α5 receptor, Lutheran (Lu) glycoprotein and its truncated form, basal cell adhesion molecule (B‐CAM). In cell adhesion experiments, Int β1 was crucial for adhesion to most of the purified human BM proteins. Lu/B‐CAM mediated adhesion to Lm‐511 together with Int α3β1, and was essential for the adhesion of hESCs to embryonic feeder cells. Adhesion to Lm‐411 was mediated by Int α6β1. Lm‐511 supported hESC growth in defined medium equally well as Matrigel. These results provide consequential information of the biological role of BM in hESCs, warranting further investigation of BM biology of human pluripotent stem cells.Sanna Vuoristo, Ismo Virtanen, Minna Takkunen, Jaan Palgi, Yamato Kikkawa, Patricia Rousselle, Kiyotoshi Sekiguchi, Timo Tuuri, and Timo Otonkoski, "Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support", Journal of Cellular and Molecular Medicine, 13, 8b, pp. 2622-2633, Wiley, 200

https://ir.library.osaka-u.ac.jp/repo/ouka/all/71822/JCMM_13_8b_2622.pdf

Laminin isoforms in human embryonic stem cells : synthesis, receptor usage and growth support

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Osaka University Knowledge Archive

Osaka University Knowledge Archive