In the last three years, five cases of pyelonephritis in the cow have been attended by the clinic staff. Several cases were reported from Humboldt County. This disease is reported as being quite common in European countries. The writer has had the opportunity to make bacteriological examination in two of the above mentioned cases and one case in the pig

Rice, C. D.

English

University of Northern Iowa

Proceedings of the Iowa Academy of Science Volume 34 Annual Issue Article 13 1927 Specific Infectious Pyelonephritis of Cows C. D. Rice Iowa State College Copyright ©1927 Iowa Academy of Science, Inc. Follow this and additional works at: https://scholarworks.uni.edu/pias Recommended Citation Rice, C. D. (1927) "Specific Infectious Pyelonephritis of Cows," Proceedings of the Iowa Academy of Science, 34(1), 87-88. Available at: https://scholarworks.uni.edu/pias/vol34/iss1/13 This Research is brought to you for free and open access by the Iowa Academy of Science at UNI ScholarWorks. It has been accepted for inclusion in Proceedings of the Iowa Academy of Science by an authorized editor of UNI ScholarWorks. For more information, please contact scholarworks@uni.edu. SPECIFIC INFECTIOUS PYELONEPHRITIS OF COWS C. D. Rrc:e In the last three years, five cases of pyelonephritis in the cow have been attended by the clinic staff. Several cases were reported from Humboldt County. This disease is reported as being quite common in European countries. The writer has had the oppor-tunity to make bacteriological examination in two of the above mentioned cases and one case in the pig. Case No. 4572. On February 25, 1925 a sample of urine from the clinic depart-ment was sent to our laboratories for examination with the follow-ing history: "Dr. F. E. \Valsh of the Ambulatory Clinic had two cases of this nature, one in 1923 one in 1924. \Ve would like. if possible, to determine the etiology. This is a valuable Holstein cow eight years old. The animal is hidebound, emaciated and weak. Exhibits painful urination. The urine is turbid and blood stained." On mounting a loopful of the non-centrifuged urine and staining, myriads of bacilli were observed with a few red cells, leucocytes, and epithelial cells. The picture resembled closely a cut in Hutyra and ::-Iarek. On looking this cut up under the head "bacillus renalis" it was found to be labelled "clumps Corynebacterium renalis." On making inoculations from the urine on to Huntoon's hormone agar pure cultures were secured. On March 2, 1925 the left kidney of the cow was removed. Pure cultures were obtained from several different parts of the organ. So far as the writer was able to ascertain by culturing and staining, the organisms were true diphtheroids, staining beautifully with Albert's stain. A few weeks later Dr. \\'alsh had the fourth case in a two year old heifer and the kidney was removed. Case Ko. 5054. On l\larch 5, 1926 a sample of urine .came from clinic for ex-amination. History: "Eight year old Holstein cow, emaciated. Passing blood-urine, calved about six or eight weeks ago." Smears from the urine revealed large numbers of cliphtheroid organ'.sms. Pure culture was obtained. The following day the left kidney was removed and pure cultures of a diphtheroid obtained from several parts of the kidney. 87 1Rice: Specific Infectious Pyelonephritis of CowsPublished by UNI ScholarWorks, 192788 IOWA ACADEMY OF SCIENCE Dr. Benbrook who was making a chemical and histopathological examination of these cases called my attention to a reprint from rt:he Journal of Experimental Medicine, November 1, 1925, by Jones and Little, describing thirteen cases from three herds of cows, purchased from the middle west and shipped to New Jersey. This is an excellent piece of research work and the first report of diseases of this nature in this country to be due to a diphtheroid organism. Summary. In the past three years the clinic at Ames has had five cases of pyelonephritis in the cow, all of which proved fatal. The disease is characterized by passing bloody urine shortly after calving. Four of these cases were bilateral. A true diphtheroid organism was secured in pure culture from the urine and kidney of two cases. The disease is present in this country and in Iowa, and due to its fatality is of economic importance. BIBLIOGRAPHY HUTYRA and MAREK, Vol. 1, p. 1018-1912 Ed. BUCHANAN, Veterinary Bact., p. 361. }ONES and LITTLE, Journal of Experimental Medicine, Nov. 1, 1925, Vol. xiii, No. 5, p. 593. BEMIS, V cterinary Medicine, Vol. XXI, No. 4, p. 161. DEPARTMENT OF VETERIXARY PATHOLOGY, low A STATE COLLEGE, AMES, low A. THE USE OF SODIUM TAUROCHOLATE AND CRYS-TAL VIOLET IN THE ISOLATION OF BACT. TUMEFACIENS SM. & TOWN. M. K.. PA'l'EL In the isolation of Bacterium t11mcfacic11s from overgrowths on apple, one of the greatest sources of contamination is that of soil organisms. These are lodged in the soil within the crevices of the convoluted gall surface. In a<ldit'.on the crown gall oraanisms . b frequently occur in only small numbers, making it necessary to use large amounts of tissue for isolation purposes. It was found that the addition of small quantities of sodium taurocholate and crystal violet to the dextrose agar inhibited large numbers of the contaminants without inhibiting Bacterium tume-facicns. 2Proceedings of the Iowa Academy of Science, Vol. 34 [1927], No. 1, Art. 13https://scholarworks.uni.edu/pias/vol34/iss1/13

Specific Infectious Pyelonephritis of Cows

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Specific Infectious Pyelonephritis of Cows

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