In the technique reported by Stiles and Eastwood (Iowa Acad. Sci. Proc. 1940) difficulty was encountered in floating Diglycol Stearate sections upon glass slides. The usual water albumen method of affixing ribbons upon slides could not be used satisfactorily for two reasons: first, because Diglycol is soluble in water, thus causing the ribbon to disintegrate before the tissue is permanently affixed to the slide and secondly because the surface tension of water causes a spreading effect of the ribbon. The difficulties of affixing the tissue to the slide when it is Diglycol embedded constituted a serious weakness in this technique. This investigation was undertaken in an attempt to find a floating agent which would not dissolve Diglycol ribbons in any appreciable amount. The use of calcium chloride as a floating agent was suggested by C. E. Moritz. Its solubility was tested in the usual manner, and it was found that Diglycol was not dissolved by it
