Plasma, serum, and body fluid specimens (pleural fluid, ascitic fluid, synovial fluid, edema fluid, hydrocele fluid, cyst fluid, lymph, bile, and subdural fluid) from patients with heart disease, carcinoma, liver disease, and arthritis, were subjected to electrophoretic analysis. A decrease in plasma albumin and an increase in globulin components occurred in the disease conditions. In most instances the albumin and gamma globulin were higher, the α1 globulin the same, and the α2 and β globulin and fibrinogen in lower concentration in the fluid than in the corresponding plasma specimen. The distribution of the protein components between the plasma and fluid is explained on the basis of their molecular weights
