Trade-off between transcriptome plasticity and genome evolution in cephalopods

Abstract

Author Posting. © The Author(s), 2017. This is the author's version of the work. It is posted here by permission of Cell Press for personal use, not for redistribution. The definitive version was published in Cell 169 (2017): 191-202, doi:10.1016/j.cell.2017.03.025.RNA editing, a post-transcriptional process, allows the diversification of proteomes beyond the genomic blueprint; however it is infrequently used among animals. Recent reports suggesting increased levels of RNA editing in squids thus raise the question of their nature and effects in these organisms. We here show that RNA editing is particularly common in behaviorally sophisticated coleoid cephalopods, with tens of thousands of evolutionarily conserved sites. Editing is enriched in the nervous system affecting molecules pertinent for excitability and neuronal morphology. The genomic sequence flanking editing sites is highly conserved, suggesting that the process confers a selective advantage. Due to the large number of sites, the surrounding conservation greatly reduces the number of mutations and genomic polymorphisms in protein coding regions. This trade-off between genome evolution and transcriptome plasticity highlights the importance of RNA recoding as a strategy for diversifying proteins, particularly those associated with neural function.NLB was supported by a post-doctoral scholarship from the Center for Nanoscience and Nanotechnology, Tel-Aviv University. The research of RU is supported by the Israel Science Foundation (772/13). The research of EYL was supported by the European Research Council (311257) and the Israel Science Foundation (1380/14). The research of JJCR was supported by the National Institutes of Health [1R0111223855, 1R01NS64259], the National Science Foundation (HRD- 1137725), and the Frank R. Lillie and Laura and Arthur Colwin Research Fellowships from the Marine Biological Laboratory in Woods Hole. The work of JJCR and EE was supported by grant No 094/2013 from the United States-Israel Binational Science Foundation (BSF).2018-04-0