Background : The result of infection control procedure survey in Puskesmas showed
that there were the health worker’s practices that increased potentially for
transmission of infection, not only for patients but also for health workers.
Sterilization is an important component for annihilating all microorganism include
spore. As a standard sterilizer, autoclave was rarely had by Puskesmas (13.3%). Press
cooker has the same principle mechanism as autoclave, therefore it can be used to
sterilizer.
Objective :The purpose of this study was to investigate the press cooker effectiveness
as sterilizer to sterilize Bacillus subtilis.
Methods : This study used laboratory experimental with posttest only with control
group design. The samples were 54 tubes of Bacillus subtilis bacteria. In first
research, the samples were 30 tubes of Bacillus subtilis. The variable was ability for
sterilizing Bacillus subtilis by press cooker in 15 minutes. In second research, the
samples were 24 tubes of Bacillus subtilis. The variable was the need of time for
sterilizing Bacillus subtilis by press cooker. The result of sterilization test for Bacillus
subtilis can be observed by incubation bacteria tubes into incubato for 24 hours in
37°C. The media were sterile, if they were transparent.
Results : The result was press cooker can sterilize all of the Bacillus subtilis (100%)
like autoclave in 15 minutes. The Kappa’s coefficient showed there was very good
coefficient (K > 0.75) between press cooker and autoclave as sterilizer. Press cooker
can sterilize Bacillus subtilis in 1 minutes. There was not the significant difference of
time between a press cooker an another for sterilizing Bacillus subtilis.
Conclusion : Press cooker had the same effectiveness as autoclave in sterilizing
Bacilus subtilis. Press cooker needs time 11 minutes for sterilizing.
Keyword : Press cooker and autoclave, Sterilize, Bacillus subtili
