Yuselis Castaño-Guerrero acknowledges Fundação para a Ciência e a Tecnologia (FCT) her PhD Grant (SFRH/BD/145590/2019)info:eu-repo/semantics/publishedVersio

Fortunato, Elvira

Guerrero, Yuselis

Moreira, Felismina

Sales, M. G. F.

English

Universidade do Minho: RepositoriUM

SERS-based biosensors for on-site determinationof pancreatic cancer biomarkersY. Castaño-Guerrero1,3, F.T.C. Moreira1,3, E. Fortunato4, M.G.F. Sales1,2,31BioMark@ISEP/, Rua Dr. António Bernandino de Almeida, 431, 4249-015, Porto, Portugal; 2BioMark@UC, Rua Sílvio Lima 3030-790, Coimbra, Portugal; 3CEB, Centre of Biological Engineering, University of Minho, Braga, Portugal;4 CENIMAT|i3N, Departamento de Ciência de Materiais, Faculdade de Ciências e Tecnologia,  Universidade NOVA de Lisboa, Lisboa, and CEMOP/UNINOVA, Campus da Caparica, Caparica, PortugalIntroductionMethodology ResultsGeneral considerationsAcknowledgments(a) Preparing biosensing supports.(b) Using plastic antibodies as biorecognition elements.(c) Enable suitable SERS detection for single analytes.Secreting cellRecipient cellCirculating EXOsPancreatic cancer is the most lethal cancer disease, as anoutcome of its late diagnosis. Biomarkers that signal thedisease include circulating vesicles as exosomes (EXOs)and their cargo, miRNA. Thus, this work aims to develop anew generation of biosensors sensitive to these EXOs andrelevant miRNAs in their cargo, based on surface enhancedRaman scattering (SERS) as transducing element. Figure 1. Exosomes and their cargo, exported from the origin cell and delivered to the target cell.300 400 500 600 700 8000.010.030.05WavelengthAbsorbancevoid@Au@mSiOPS@AuNP@mSiO void@AuNP@mSiOFigure 2. Schematic representation of the operation of the SERS platform to detect exosomal biomarkers.1000 1050 1100 1150051525Raman shiftRaman Intensity 100 ng/ml3 ng/ml0.3 ng/mlYuselis Castaño-Guerrero acknowledges Fundação para a Ciência e aTecnologia (FCT) her PhD Grant (SFRH/BD/145590/2019).200 600 10000100200300Calibration MIPZ´(Ω)−Z´´(Ω)PhB0.1 ng/ml0.3 ng/ml1 ng/ml3 ng/ml10 ng/ml31 ng/ml100 ng/mlnanocapsulevoid@AuNP(growth1)@mSiOSERS detection employs metal nanostructures that may enhance thesignal. Different surface protein biomarkers in pancreatic cancer areexplored to this end. Different metal nanostructures are used andtested to carry the Raman reporter. Simple or mixed standardsolutions may be employed following this approach.Calibration y Nyquist plotsRaman spectra

SERS-based biosensors for on-site determination of pancreatic cancer biomarkers

https://repositorium.sdum.uminho.pt/bitstream/1822/77517/1/document_55536_1.pdf

SERS-based biosensors for on-site determination of pancreatic cancer biomarkers

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