Review on AKAP9 (A kinase (PRKA) anchor protein (yotiao) 9), with data on DNA, on the protein encoded, and where the gene is implicated

Ciampi, Raffaele

Nikiforov, Yuri E.

Atlas of Genetics and Cytogenetics in Oncology and Haematology

I-Revues

  
 
 
 
 
Gene Section 
Short Communication 
 
Atlas Genet Cytogenet Oncol Haematol. 2007;11(4)  264 
Atlas of Genetics and Cytogenetics 
in Oncology and Haematology                         
                         OPEN ACCESS JOURNAL AT INIST-CNRS 
AKAP9 (A kinase (PRKA) anchor protein (yotiao) 
9) 
Raffaele Ciampi, Yuri E Nikiforov 
Dip. di Endocrinologia e Metabolismo, Universitá di Pisa, via Paradisa, 2, 56124 Pisa, Italy 
Published in Atlas Database: April 2007 
Online updated version: http://AtlasGeneticsOncology.org/Genes/AKAP9ID42999ch7q21.html  
DOI: 10.4267/2042/16955 
This work is licensed under a Creative Commons Attribution-Non-commercial-No Derivative Works 2.0 France Licence. 
© 2007 Atlas of Genetics and Cytogenetics in Oncology and Haematology 
 
Identity 
Hugo: AKAP9 
Other names: AKAP350; AKAP450; CG-NAP; 
HYPERION; KIAA0803; MU-RMS-40.16A; PRKA9; 
YOTIAO 
Location: 7q21.2 
DNA/RNA 
Description 
AKAP9 gene consists of 51 encoding exons with a total 
gene size of 169797 bp. 
Transcription 
The AKAP9 transcript has an open reading frame 
(ORF) of 11724 bp. Four different transcript variants 
have been identified (NM147171, NM005751, 
NM147185, NM147166). 
Protein 
Description 
The 11.7 kb ORF encodes for a 3908 aa protein with a 
predicted size of 453 kDa. Four different protein 
products have been described (NP005742.4, 
NP671695.1, NP671700.1, NP671714.1). 
Localisation 
Centrosomes, Golgi apparatus. 
Function 
AKAP9 belongs to the family of A-Kinase Anchor 
Proteins (AKAPs), which are scaffold proteins able to 
bind the type II regulatory subunit (RII) of cAMP 
dependent Protein Kinase A (PKA) and several other 
protein kinases and phosphatases and to anchor them to 
specific intracellular compartments. AKAP9 shows 
centrosome and Golgi compartmentalization. Various 
splice variants of the transcript are found in different 
human tissues. The AKAP9 protein contains the PKA 
binding domain, a large coiled-coil domain and C-
terminal centrosome binding domain. 
Implicated in 
Papillary Thyroid Carcinoma (PTC) 
Cytogenetics 
inv(7)(q21-22q34) with AKAP9-BRAF fusion. 
Breakpoints 
Note: Breakpoint in AKAP9-BRAF fusion is located 
within intron 8 of the gene. In this fusion, exons 1-8 of 
AKAP9 are fused with last 10 exons 9-18 of BRAF. In 
the fusion, AKAP9 lacks the centrosome binding 
domain and, as a result, the AKAP9-BRAF protein 
looses its cytoplasmic compartmentalization and 
appears to be diffusely distributed in the cytoplasm, as 
detected by immunofluorescence. 
References 
Schmidt PH, Dransfield DT, Claudio JO, Hawley RG, Trotter 
KW, Milgram SL, Goldenring JR. AKAP350, a multiply spliced 
protein kinase A-anchoring protein associated with 
centrosomes. J Biol Chem 1999;274(5):3055-3066. 
Takahashi M, Shibata H, Shimakawa M, Miyamoto M, Mukai 
H, Ono Y. Characterization of a novel giant scaffolding protein, 
CG-NAP, that anchors multiple signaling enzymes to 
centrosome and the golgi apparatus. J Biol Chem 
1999;274(24):17267-17274. 
Witczak O, Skǻlhegg BS, Keryer G, Bornens M, Taskén K, 
Jahnsen T, Orstavik S. Cloning and characterization of a cDNA 
encoding an A-kinase anchoring protein located in the 
centrosome, AKAP450. EMBO J 1999;18:1858-1868. 
Edwards AS, Scott JD. A-kinase anchoring proteins: protein 
kinase A and beyond. Curr Opin Cell Biol 2000;12(2):217-221. 
AKAP9 (A kinase (PRKA) anchor protein (yotiao) 9) Ciampi R, Nikiforov YE 
 
 
 
 
 
Atlas Genet Cytogenet Oncol Haematol. 2007;11(4)  265 
Gillingham AK, Munro S. The PACT domain, a conserved 
centrosomal targeting motif in the coiled-coil proteins AKAP450 
and pericentrin. EMBO Rep 2000;1(6):524-529. 
Takahashi M, Yamagiwa A, Nishimura T, Mukai H, Ono Y. 
Centrosomal proteins CG-NAP and kendrin provide 
microtubule nucleation sites by anchoring gamma-tubulin ring 
complex. Mol Biol Cell 2002;13(9):3235-3245. 
 
 
 
Ciampi R, Knauf JA, Kerler R, Gandhi M, Zhu Z, Nikiforova  
MN, Rabes HM, Fagin JA, Nikiforov YE. Oncogenic AKAP9-
BRAF fusion is a novel mechanism of MAPK pathway 
activation in thyroid cancer. J Clin Invest 2005;115:94-101. 
This article should be referenced as such: 
Ciampi R, Nikiforov YE. AKAP9 (A kinase (PRKA) anchor 
protein (yotiao) 9). Atlas Genet Cytogenet Oncol 
Haematol.2007;11(4):264-265. 
 
 


AKAP9 (A kinase (PRKA) anchor protein (yotiao) 9)

http://documents.irevues.inist.fr/bitstream/2042/16955/5/04-2007-AKAP9ID42999ch7q21.pdf

AKAP9 (A kinase (PRKA) anchor protein (yotiao) 9)

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