National Foundation for Science and Technology, I.P., FCT, with reference SFRH/BD/130107/2017info:eu-repo/semantics/publishedVersio

Cardoso, Rita

Fortunato, Elvira

Sales, M. G. F.

English

Universidade do Minho: RepositoriUM

Driving nanozymes towards stereochemical recognition: application to biomolecules of interest in healthAna Rita A. Cardoso1,3,4, Elvira Fortunato4,  M. Goreti F. Sales1,2,3IntroductionMethodology and Results Main Goals National Foundation for Science and Technology, I.P., FCT, with reference SFRH/BD/130107/2017. 1 BioMark@ISEP, School of Engineering, Polytechnic Institute of Porto, Porto, Portugal2 BioMark@UC, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal3 CEB, Centre of Biological Engineering, University of Minho, Braga, Portugal4 CENIMAT|i3N, Departamento de Ciência de Materiais, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Lisboa, and CEMOP/UNINOVA, Campus da Caparica, Caparica, PortugalA BElectrical StimulusC D LEGEND:Metal Nanoparticles Analyte Cross-linker MonomerEnzymes are used in many biotechnological approaches due to their excellent catalytic properties and their ability to recognize a specifictarget compound within a complex matrix. As an alternative, artificial catalytic systems have emerged to replace natural enzymes. However,these systems use nanostructured materials that can catalyze a reaction but lack the necessary stereochemical recognition capability. Herein,catalytic materials with molecularly imprinted materials (MIP) emerge to make these artificially catalyzed reactions a spatial recognitionevent. A very important system that uses enzymes is the glucose meter, the first line of glucose control in diabetic patients. An innovativeenzyme-free glucose biosensor is developed here by using nanozymes and MIPs. Considering the current pandemics, the proposed technicalapproach has been extended to detect human antibodies against SARS-CoV-2.(i) Targeting Glucose and antibodies against SARS-CoV-2, by means of:• preparing an artificial material acting like an enzyme; • applying it to prepare a suitable electrochemical sensor (to produce analytical features that may compete with current commercially available devices);• enabling a colour read-out system by employing suitable nanostuctured materials. (ii) Analysing real samples and validating results against the currently certified methods of analysis.(A) Use carbon supports made of 3-electrodes printed on a substrate(B) Have metal nanoparticles on the sensing layer(C) Have suitable monomers and cross-linkers to have MIPs prepared(D) Remove the target analyte and check the responseElectrochemical Measurements to follow each step of the assembly-1500150-0.3 0.2 0.7I(µA)Potential (V)After Cleaning Electrodeposition of metal nanoparticles MIP NIP0300600450 1700 2950-Z''(Ω)Z'(Ω)03570-0.3 0.2 0.7I(µA)Potential (V) -1000100-0.3 0.2 0.7I(µA)Potential (V) MIP NIP Removal MIP Removal NIP0300600500 1250 2000-Z''(Ω)Z'(Ω)02040-0.3 0.2 0.7I(µA)Potential (V)-1500150-0.3 0.2 0.7I(µA)Potential (V)After Cleaning Electrodeposition of metal nanoparticles MIP NIP0300600450 1700 2950-Z''(Ω)Z'(Ω)03570-0.3 0.2 0.7I(µA)Potential (V) -1000100-0.3 0.2 0.7I(µA)Potential (V) MIP NIP Removal MIP Removal NIP0300600500 1250 2000-Z''(Ω)Z'(Ω)02040-0.3 0.2 0.7I(µA)Potential (V)A.R. Cardoso, M.F. Frasco, E. Fortunato, M. Goreti, F. Sales, Molecular Imprinting on Nanozymes for Sensing Applications, Biosensors. 11 (2021) 1–19. PublicationAcknowledgment

Driving nanozymes towards stereochemical recognition: application to biomolecules of interest in health

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Driving nanozymes towards stereochemical recognition: application to biomolecules of interest in health

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