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Determination of isoflavone glucoside malonates in Trifolium pratense L. (red clover) extracts: quantification and stability studies
Authors
F. Ariese
U.A.T. Brinkman
+3 more
E. Drs. de Rijke
C. Gooije
A. Zafra-Gomez
Publication date
1 January 2001
Publisher
Doi
Cite
Abstract
Isoflavones, their glucosides and their glucoside malonates were determined in red clover leaf extracts using reversed-phase LC coupled to atmospheric pressure chemical ionisation mass spectrometry (APCI-MS), UV and fluorescence detectors and the stability of the malonates was investigated. Extracts can be stored at least 1-2 weeks at -20°C without loss of malonates. In LC-separated fractions the malonates are most stable when stored at low temperature after evaporation to dryness. The concentrations of eight major isoflavones ranged from 0.04 to 5 mg/g leaves. © 2001 Elsevier Science B.V. All rights reserved
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