Brozel, Volker

Clay, Sharon A.

Gibson, Susan

Hoese, Aaron

English

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South Dakota State UniversityOpen PRAIRIE: Open Public Research Access InstitutionalRepository and Information ExchangeAgronomy, Horticulture and Plant Science FacultyPublicationsDepartment of Agronomy, Horticulture, and PlantScience2005The Influence of Antimicrobial Chemicals onHerbicide Degrading OrganismsAaron HoeseSouth Dakota State UniversitySusan GibsonSouth Dakota State UniversityVolker BrozelSouth Dakota State University, volker.brozel@sdstate.eduSharon A. ClaySouth Dakota State University, sharon.clay@sdstate.eduFollow this and additional works at: https://openprairie.sdstate.edu/plant_faculty_pubsThis Abstract is brought to you for free and open access by the Department of Agronomy, Horticulture, and Plant Science at Open PRAIRIE: OpenPublic Research Access Institutional Repository and Information Exchange. It has been accepted for inclusion in Agronomy, Horticulture and PlantScience Faculty Publications by an authorized administrator of Open PRAIRIE: Open Public Research Access Institutional Repository andInformation Exchange. For more information, please contact michael.biondo@sdstate.edu.Recommended CitationHoese, Aaron; Gibson, Susan; Brozel, Volker; and Clay, Sharon A., "The Influence of Antimicrobial Chemicals on HerbicideDegrading Organisms" (2005). Agronomy, Horticulture and Plant Science Faculty Publications. 185.https://openprairie.sdstate.edu/plant_faculty_pubs/185362 Proceedings of the South Dakota Academy of Science, Vol. 84 (2005)THE INFLUENCE OF ANTIMICROBIALCHEMICALS ON HERBICIDEDEGRADING ORGANISMSAaron Hoese, Susan Gibson, Volker Brozel and Sharon A. ClayPlant ScienceSouth Dakota State UniversityBrookings, SD 57007ABSTRACT Tylosin (Tyl) and chlortetracycline (CTC) are antimicrobial chemicals used as growth promoters in cattle, swine, and poultry production and can be excreted as the parent compound.  Landspreading manure can move these chemicals into soil and thereby change the soil microbial make up or degradative activity.  The objective of this study was to determine if Tyl and CTC changed the growth or herbicide degrading activity of pure cultures of two bacteria, Pseudomonas ADP, an atrazine degrader, and Sphingobium herbicidovorans, a 2,4-D degrader.  The P. ADP was cultured in liquid minimal broth with atrazine as the sole N source for 5 d.  Tyl or CTC was added to the media at 2.5 ppm.  The culturable count was determined every 24 h.  The activity of the S. herbicidovorans was measured over 14 d by placing 14C-carboxy-labeled 2,4-D in a biometer flask and adding a 5 ml solution containing  3.7 x 109 CFU of the degrader.  14CO2 generated by 2,4-D degradation was trapped in NaOH and radioactivity quantified by liquid scintil-lation counting.  P. ADP in the control media grew well during the incubation. Tyl inhibited P. ADP temporarily and recovery occurred within 48 h.  The CTC treatment killed P. ADP, as the culturable count was below the detection limit by 72 h.  About 88% of the 2,4-D in control treatments was mineralized by S. herbicidovorans 4 d after treatment (DAT), whereas Tyl slowed the initial deg-radation with only 4% mineralized by 10 DAT.  By 14 DAT, recovery occurred and 2,4-D mineralization in the Tyl treatment was similar to the control.  CTC substantially decreased the activity of S. herbicidovorans with only 12% of the added 2,4-D mineralized 14 DAT.

The Influence of Antimicrobial Chemicals on Herbicide Degrading Organisms

https://openprairie.sdstate.edu/cgi/viewcontent.cgi?article=1174&context=plant_faculty_pubs

The Influence of Antimicrobial Chemicals on Herbicide Degrading Organisms

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