We have combined a laser scissors and a laser tweezers to study, (1) the response of nerve fiber growth cones to laserinduced damage on single axons, and (2) localized microfluidic flow generated by laser-driven spinning birefringent particles. In the laser scissors study, sub-axotomy damage elicits a growth cone response whether damage is on the same or an adjacent axon. In laser tweezers study, the axon growth cones turn in response to the optically driven microfluidic flow. In summary, both the laser scissors and the laser tweezers studies elicit growth cone turning responses
