Impact of extracellular vesicles of Staphylococcus aureus N305 on the immune response of the host. STLOpenday

SILVA ROSA DA LUZ, Brenda

English

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Submitted on 5 Jun 2020
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Impact of extracellular vesicles of Staphylococcus aureus
N305 on the immune response of the host
Brenda Silva Rosa da Luz
To cite this version:
Brenda Silva Rosa da Luz. Impact of extracellular vesicles of Staphylococcus aureus N305 on the
immune response of the host. STLOpendays, Institut National de Recherche Agronomique (INRA).
UMR UMR INRA / AgroCampus Rennes : Science et Technologie du Lait et de l’?uf (1253)., Mar
2019, Rennes, France. ￿hal-02098690￿
Bacterial extracellular vesicles and health: 
positive and negative effects
STLOpen Days
19-21 March 2019
Brenda LUZ – Doctorate
MicroBio - Eric GUEDON / Yves LE LOIR
STLOpen Days
19-21 March 2019
Bacterial extracellular 
vesicles and health: 
positive and negative 
effects
Health & wellbeing
Develop functional and 
designer food against modern 
lifestyle diseases
Animal health
Offer alternatives to antibiotic
treatments (probiotics)
STLOpen Days
19-21 March 2019
Introduction
What are Extracellular Vesicles (EVs)?
 Spherical, lipid bilayer nanoparticles 
(30 – 500 nm)
 Produced by any living cell
Archaea, Bacteria and Eukaryotes
 Carry various bioactive molecules
- Proteins, nucleic acids, lipids, metabolites
- Delivered to local or distant cellular targets: interaction/modification
 Considered a mechanism of cellular intercommunication
STLOpen Days
19-21 March 2019
Introduction
Extracellular Vesicles (EVs)
 Emerging field
1966
Gram-negative EVs
Knox et al. ; Work et al.
2009
Gram-positive EVs
Lee et al.
STLOpen Days
19-21 March 2019
Introduction
Extracellular Vesicles (EVs)
 Emerging field
1966
Gram-negative EVs
Knox et al. ; Work et al.
2009
Gram-positive EVs
Lee et al.
2018
Staphylococcus aureus 
NewBould305 EVs 
Tartaglia et al.
2018
Propionibacterium 
freudenreichii EVs 
Vinicius et al.
STLOpen Days
19-21 March 2019
Introduction
What do bacterial EVs do?
(Adapted - Haurat, Elhenawy, & Feldman, 2015)
Offense
Defense
STLOpen Days
19-21 March 2019
Introduction
Our research aims:
(Prado-Rosales et al, 2014; Lee et al., 2009)
Biological Roles
WHY
Communication intra- and inter-species 
Group behavior and community functioning
Interactions with microbiota and host
Nucleus
Characterization
WHO/WHAT
Production (species, strains…)
Compositions (proteins, nucleic acids, …)
Biogenesis
WHEN/WHERE/HOW
Formation mechanisms
Content selection mechanisms
STLOpen Days
19-21 March 2019
Introduction
Bacterial EVs and health
Propionibacterium 
freudenreichii
Staphylococcus 
aureus
Probiotic 
Properties
Virulence 
Properties
Our research aims:
Characterization
WHO/WHAT
Biological Roles
WHY
Biogenesis
WHEN/WHERE/HOW Immunomodulation
STLOpen Days
19-21 March 2019
Bacterial EVs and health: positive effects
Propionibacterium freudenreichii
 Probiotic bacterium – role  in cheese production
 Anti-inflammatory properties
 SlpB surface protein being carried by EVs
(Deutsch et. al, 2017)
Swiss-type cheeseGram-positive
Role of EVs in the probiotic properties of P. freudenreichii?
Host-cellEVsP. freudenreichii
STLOpen Days
19-21 March 2019
Bacterial EVs and health
Staphylococcus aureus
 Medical and veterinary importance
 It is one of the most important etiological agent of mastitis
Gram-positive
Inflammation of the mammary gland
- Production disease in dairy herds
- Affects animal health and milk quality
- Treatment is still ineffective
STLOpen Days
19-21 March 2019
Bacterial EVs and health: negative effects
Staphylococcus aureus EVs
 Strain N305 – bovine  mastitis isolate
- Induce a pro-inflammatory response expression in vitro
(Tartaglia et al., Front Cell Infect Microbiol, 2018)
Living N305 HK N305 LTAPBS EVs 1 µg EVs 10 µg
- Promote tissue inflammation in vivo
How do EVs from S. aureus 
N305 strain impact the 
immune response of the host? 
STLOpen Days
19-21 March 2019
Perspectives
 Expand the knowledge about EVs produced by Gram-positive bacteria
 Improve the understanding of the interactions of EVs with host cells
 Provide molecular and functional data for the use of EVs as biotechnological agents
- New vesicular systems for therapy
- Development of vaccines or drugs for prevention of diseases
- Delivery of bioactive molecule (immunomodulating compounds)
13Please visit http://www.rennes.inra.fr/stlo_engSTLOpen Days
19-21 March 2019
THANK YOU
MERCI


Impact of extracellular vesicles of Staphylococcus aureus N305 on the immune response of the host

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Impact of extracellular vesicles of Staphylococcus aureus N305 on the immune response of the host

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