Escherichia coli sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene bla , is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing E. coli isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and bla . A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i) bla negative non-ST131, (ii) bla positive non-ST131, (iii) bla negative ST131, or (iv) bla positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited bla , whereas 165 (47%) were ST131. ST131 accounted for 56% of bla -positive-versus 35% of bla -negative isolates (

Ari Robicsek

Brian D. Johnston

Carl Urban

Clermont

Clinical Laboratory Standards Institute

Connie Clabots

Coque

David Paterson

Ender

Glen Hansen

James H. Jorgensen

James R. Johnson

James S. Lewis

Javier Adachi

John Quinn

Johnson

Leflon-Guibout

Lewis

McGettigan

Michael A. Kuskowski

Nancy D. Hanson

Nicolas-Chanoine

Paul H. Edelstein

Peakall

Peirano

Pitout

Ribot

Rogers

Russo

Scott J. Weissman

Sidjabat

Tenover

Timothy Cleary

Urban

Vigil

PubMed

ABSTRACT
          
            Escherichia coli
            sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene
            bla
            CTX-M-15
            , is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing
            E. coli
            isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and
            bla
            CTX-M-15
            . A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i)
            bla
            CTX-M-15
            negative non-ST131, (ii)
            bla
            CTX-M-15
            positive non-ST131, (iii)
            bla
            CTX-M-15
            negative ST131, or (iv)
            bla
            CTX-M-15
            positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited
            bla
            CTX-M-15
            , whereas 165 (47%) were ST131. ST131 accounted for 56% of
            bla
            CTX-M-15
            -positive- versus 35% of
            bla
            CTX-M-15
            -negative isolates (
            P
            &lt; 0.001). Whereas ST131 accounted for 94% of the 175 total group B2 isolates, non-ST131 isolates were phylogenetically distributed by
            bla
            CTX-M-15
            status, with groups A (
            bla
            CTX-M-15
            -positive isolates) and D (
            bla
            CTX-M-15
            -negative isolates) predominating. Both
            bla
            CTX-M-15
            and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non-ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles.
            bla
            CTX-M-15
            was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus,
            E. coli
            ST131 and
            bla
            CTX-M-15
            are emergent, widely distributed, and predominant among ESBL-positive
            E. coli
            strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats.
          </jats:p

null null

Crossref

Antimicrobial Agents and Chemotherapy

Molecular Epidemiological Analysis of Escherichia coli Sequence Type ST131 (O25:H4) and
            <i>bla</i>
            <sub>CTX-M-15</sub>
            among Extended-Spectrum-β-Lactamase-Producing E. coli from the United States, 2000 to 2009

Escherichia coli
sequence type ST131 (from phylogenetic group B2), often carrying the extended-spectrum-β-lactamase (ESBL) gene
bla
CTX-M-15
, is an emerging globally disseminated pathogen that has received comparatively little attention in the United States. Accordingly, a convenience sample of 351 ESBL-producing
E. coli
isolates from 15 U.S. centers (collected in 2000 to 2009) underwent PCR-based phylotyping and detection of ST131 and
bla
CTX-M-15
. A total of 200 isolates, comprising 4 groups of 50 isolates each that were (i)
bla
CTX-M-15
negative non-ST131, (ii)
bla
CTX-M-15
positive non-ST131, (iii)
bla
CTX-M-15
negative ST131, or (iv)
bla
CTX-M-15
positive ST131, also underwent virulence genotyping, antimicrobial susceptibility testing, and pulsed-field gel electrophoresis (PFGE). Overall, 201 (57%) isolates exhibited
bla
CTX-M-15
, whereas 165 (47%) were ST131. ST131 accounted for 56% of
bla
CTX-M-15
-positive- versus 35% of
bla
CTX-M-15
-negative isolates (
P
< 0.001). Whereas ST131 accounted for 94% of the 175 total group B2 isolates, non-ST131 isolates were phylogenetically distributed by
bla
CTX-M-15
status, with groups A (
bla
CTX-M-15
-positive isolates) and D (
bla
CTX-M-15
-negative isolates) predominating. Both
bla
CTX-M-15
and ST131 occurred at all participating centers, were recovered from children and adults, increased significantly in prevalence post-2003, and were associated with molecularly inferred virulence. Compared with non-ST131 isolates, ST131 isolates had higher virulence scores, distinctive virulence profiles, and more-homogeneous PFGE profiles.
bla
CTX-M-15
was associated with extensive antimicrobial resistance and ST131 with fluoroquinolone resistance. Thus,
E. coli
ST131 and
bla
CTX-M-15
are emergent, widely distributed, and predominant among ESBL-positive
E. coli
strains in the United States, among children and adults alike. Enhanced virulence and antimicrobial resistance have likely promoted the epidemiological success of these emerging public health threats

Johnson, James R

Urban, Carl

Weissman, Scott J

Jorgensen, James H

Lewis, James S

Hansen, Glen

Edelstein, Paul H

Robicsek, Ari

Cleary, Timothy

Adachi, Javier

Paterson, David

Quinn, John

Hanson, Nancy D

Johnston, Brian D

Clabots, Connie

Kuskowski, Michael A

AMERECUS Investigators

University of Miami: Scholarship@Miami

Molecular Epidemiological Analysis of Escherichia coli Sequence Type ST131 (O25:H4) and blaCTX-M-15 among Extended-Spectrum-β-Lactamase-Producing E. coli from the United States, 2000 to 2009

Johnson, James R.

Urban, Carl

Weissman, Scott J.

Jorgensen, James H.

Lewis, James S.

Hansen, Glen

Edelstein, Paul H.

Robicsek, Ari

Cleary, Timothy

Adachi, Javier

Paterson, David

Quinn, John

Hanson, Nancy D.

Johnston, Brian D.

Clabots, Connie

Kuskowski, Michael A.

University of Queensland eSpace

Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009

http://espace.library.uq.edu.au/view/UQ:274434/UQ274434_OA.pdf

Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and bla CTX-M-15among extended-spectrum-β- lactamase-producing E. coli from the United States, 2000 to 2009

Abstract

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Crossref

University of Miami: Scholarship@Miami

University of Queensland eSpace