Amatya, Shaili

Amin, Asma

Hsu, Leewen

Parton, Lance A

Paudel, Umesh

Rajbhandari, Sharina

Zylak, Anna

English

The Touro College and University System

Touro Scholar NYMC Faculty Posters Faculty Spring 3-23-2017 ROCK2 Gene Single Nucleotide Polymorphisms and Association with Bronchopulmonary Dysplasia in Extremely Low Birth Weight Infants Sharina Rajbhandari New York Medical College Asma Amin New York Medical College Shaili Amatya New York Medical College Leewen Hsu New York Medical College Anna Zylak New York Medical College See next page for additional authors Follow this and additional works at: https://touroscholar.touro.edu/nymc_fac_posters  Part of the Enzymes and Coenzymes Commons, and the Respiratory Tract Diseases Commons Recommended Citation Rajbhandari, S., Amin, A., Amatya, S., Hsu, L., Zylak, A., Paudel, U., & Parton, L. A. (2017). ROCK2 Gene Single Nucleotide Polymorphisms and Association with Bronchopulmonary Dysplasia in Extremely Low Birth Weight Infants. Retrieved from https://touroscholar.touro.edu/nymc_fac_posters/26 This Poster is brought to you for free and open access by the Faculty at Touro Scholar. It has been accepted for inclusion in NYMC Faculty Posters by an authorized administrator of Touro Scholar.For more information, please contact touro.scholar@touro.edu. Authors Sharina Rajbhandari, Asma Amin, Shaili Amatya, Leewen Hsu, Anna Zylak, Umesh Paudel, and Lance A. Parton This poster is available at Touro Scholar: https://touroscholar.touro.edu/nymc_fac_posters/26 ROCK2 Gene Single Nucleotide Polymorphisms and Association with Bronchopulmonary Dysplasia in Extremely Low Birth Weight Infants    Sharina Rajbhandari, Asma Amin, Shaili Amatya, Leewen Hsu, Anna Zylak, Umesh Paudel, Lance A. Parton Neonatology, Maria Fareri Children’s Hospital at Westchester Medical Center and New York Medical College,  Valhalla, NY, United States   Background Rho associated coiled-coil containing protein kinase 2 (ROCK-2) gene • On chromosome 2p24 • ROCK: Serine/threonine protein kinase Fig 2.  ROCK-2 functional domains ROCK-2 SNP rs2290156 •  In intron; MAF (Minor Allele Frequency), C= 0.30 •  G allele and GC genotype more in RDS   ROCK-2 SNP rs726843 • In intron; MAF, A = 0.35 •TC genotype more in RDS compared to controls  ROCK-2 SNP rs978906 •  In 3’ UTR; MAF, C = 0.39 •  Affects ROCK2 expression by interfering with microRNA-1183 binding •  Association with stiffer arteries and with high altitude essential hypertension Hypothesis We hypothesize that ROCK2 gene SNP variants rs2290156, rs726843 and rs978906 are associated with development of BPD in ELBW infants. Methods Inclusion criteria • ELBW infants (birth weight < 1kg) • Informed parental consent SNP Analysis DNA was isolated from buccal swabs of 137 ELBW infants and analyzed via real-time PCR using Taqman probes for ROCK2 gene SNP variants rs2290156, rs726843 and rs978906.  BPD was defined by the need for Oxygen supplementation at 36 weeks postmenstrual age. Statistics Chi-square test, Fisher’s exact test, Mann-Whitney Rank Sum test and t-test were performed for statistical analysis; p <0.05 was considered significant. Results   No BPD (n = 57) BPD (n = 80) p value Gestational age, wks, median (IQR) 26 (24, 27) 25 (24, 26) 0.22 Birth weight, g, mean (SD) 792.9 (123.8) 752.0 (147.3) 0.09 Female Gender, n (%) 32 (56) 48 (60) 0.78 Race, n (%) Non Hispanic White 18 (33) 29 (38) 0.89 Non Hispanic Black 16 (29) 20 (26) Hispanic 16 (29) 23 (30) Other 5 (9) 5 (6) Antenatal steroids, n (%) 43 (81) 68 (91) 0.18 Genotype Distributions Genotype No BPD n (%) BPD n (%) P  value rs2290156 Wild allele  25 (64) 32 (71) 0.13 Heterozygous 7 (18) 11 (24) Minor allele 7 (18) 2 (4) GG 25 (64) 32 (71) 0.64  Any c 14 (36) 13 (28) rs726843 Wild allele  21 (55) 24 (42) 0.42 Heterozygous 12 (32) 25 (44) Minor allele 5 (13) 8 (14) GG 21 (55) 24 (42) 0.22  Any a 17 (45) 33 (58) rs978906 Wild allele  22 (52) 20 (33) 0.09 Heterozygous 11 (26) 28 (47) Minor allele 9 (21) 12 (20) TT 22 (52) 20 (33) 0.04*   Any c 20 (47) 40 (67) Conclusions • ROCK2 gene SNP rs978906 shows association with BPD  • We speculate that this variant may play a role in the development of BPD by influencing smooth muscle tone in the pulmonary vasculature References Demographic Characteristics • Liao YC, et al. Two functional polymorphisms of ROCK2 enhance arterial stiffening through inhibiting its activity and expression. Journal of Molecular and Cellular Cardiology. 2015; 79: 180–186 • Noma K, Kihara Y, Higashi Y. Striking crosstalk of ROCK signaling with endothelial function. Journal of Cardiology. 2012; 60:1–6  • Kaya G, et al. Association of Rho-kinase Gene Polymorphisms with Respiratory Distress Syndrome in Preterm Neonates. Pediatrics and Neonatology. 2017; 58: 36-42  • Pandey P, et al. Polymorphisms and haplotype of ROCK2 associated with high altitude essential hypertension in native high altitude Ladakhi Indian population: a preliminary study. Clin Exp Hypertens. 2016;38(2):238-44 Bronchopulmonary dysplasia (BPD) leads to significant morbidity in ELBW infants. Fig 1. Pathogenesis of BPD Fig 3. ROCK pathways (eNOS, endothelial Nitric Oxide Synthase; ERM, ezrin/radixin/moesin; MLCPh, Myosin Light Chain Phosphatase) *ROCK inhibitor decreases pulmonary edema, microvascular permeability and lung injury 

ROCK2 Gene Single Nucleotide Polymorphisms and Association with Bronchopulmonary Dysplasia in Extremely Low Birth Weight Infants

https://touroscholar.touro.edu/cgi/viewcontent.cgi?article=1028&context=nymc_fac_posters

ROCK2 Gene Single Nucleotide Polymorphisms and Association with Bronchopulmonary Dysplasia in Extremely Low Birth Weight Infants

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