This publication provides an overview of the long-term forest ecosystem project at the Holt Research Forest in Arrowsic, Maine. It is based on nearly 16 years of work by an interdisciplinary team from the College of Natural Sciences, Forestry, and Agriculture, including faculty, professional staff, visiting scientists, University of Maine graduate students, and undergraduate field assistants. We hope this publication will be useful to other researchers, to our workshop participants, and to others interested in forest ecosystem science.https://digitalcommons.library.umaine.edu/aes_miscpubs/1029/thumbnail.jp