Chungen, Wen

English

University of Maine

  Molecular cloning and expression of peptidoglycan recognition protein (PGRPs) gene from freshwater mussel Hyriopsis cumingii        Bao-qing Hu1, Ming-yu Zeng1, Wen-juan Dai1, Chun-gen Wen1, Yi Liu2 1. School of Life Sciences, Nanchang University, Nanchang 330031, China 2. College of Life Sciences, Jiangxi Normal University, Nanchang 330022, Jiangxi, China   ABSTRACT  Peptidoglycan recognition proteins (PGRPs) are innate immune molecules which are structurally conserved through evolution in both invertebrate and vertebrate animals. In this paper, we reported the identification and characterization of two short (HcPGRP-S1 and HcPGRP-S2) and a long (HcPGRP-L) forms of PGRP from freshwater mussel Hyriopsis cumingii. The deduced amino acid sequences of HcPGRP-S1, HcPGRP-S2 and HcPGRP-L encoded 218, 285 and 420 amino acids, respectively, and contained the conserved PGRP domain. The multiple sequences alignment showed that the amino acids sites which could bind with Zn2+ and maintain amidase activity were partly conserved in HcPGRP-S1 and HcPGRP-L, but highly conserved in HcPGRP-S2. Real-time quantitative PCR analysis showed that the trend of expression patterns were from high to low in hepatopancreas, gill, mantle, adductor muscle and hemocytes of H. cumingii. The highest level of expression quantity of HcPGRP-S1, HcPGRP-L and HcPGRP-S2 mRNA in hepatopancreas was 6.61, 11.29 and 4.95 times as the lowest level of expression quantity in hemocytes, respectively. After PGN and Aeromonas hydrophila stimulation, the expression of these genes had significant difference in tissues of hepatopancreas, hemocytes and gill except for HcPGRP-L in hemocytes. These results suggested HcPGRP could involved in the immune recognition of H. cumingii and against gram-negative bacteria infection.  Keywords: Hyriopsis cumingii, PGRPs, Gene cloning, mRNA expression                                               Corresponding author: Tel.: +86-0791-3969530; fax: +86-0791-3969530. E-mail address: cgwen@ncu.edu.cn (CG, Wen)  

Molecular cloning and expression of peptidoglycan recognition protein  gene from freshwater mussel Hyriopsis cumingii

https://digitalcommons.library.umaine.edu/cgi/viewcontent.cgi?article=1131&amp;context=isfsi

Molecular cloning and expression of peptidoglycan recognition protein gene from freshwater mussel Hyriopsis cumingii

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